Venflon pro

Manufactured by BD

Sourced in Sweden

The BD Venflon Pro is an intravenous (IV) cannula used for the administration of fluids, medications, and other medical treatments. It features a flexible plastic tube with a sharp needle for insertion into a vein.

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Lab products found in correlation

S monovette, by Sarstedt (3 mentions) Rapidpoint 500, by Siemens (1 mentions) Fentanyl, by Johnson & Johnson (1 mentions) Artflon, by BD (1 mentions) S monovette 2.7 ml ke, by Sarstedt (1 mentions) Edta containing tubes, by Sarstedt (1 mentions) S monovette k3 edta, by Sarstedt (1 mentions)

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Venflon (27 citations)

9 protocols using venflon pro

Rodent Anesthesia and Monitoring Protocol

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Twenty-four hours after LPS administration, rats were sedated with a mixture of ketamine (90 mg/kg; Alfasan, Woerden, the Netherlands), dexmedetomidine (0.125 mg/kg; Pfizer Animal Health B.V., Capelle a/d IJssel, the Netherlands) and atropine (50 µg/kg; Pharmachemie, Haarlem, the Netherlands) intraperitoneally. A 22G catheter (Venflon Pro, Becton Dickinson, Helsingborg, Sweden) was placed in the tail vein for maintenance anesthesia which consisted of ketamine (50 mg/kg/h) and dexmedetomidine (15 µg/kg/h). Lactated Ringer (Baxter BV, Utrecht, the Netherlands) was administered as maintenance fluid at a rate of 2.5 ml/kg/h.
A 22G catheter (Venflon Pro, Becton Dickinson, Helsingborg, Sweden) was placed in the carotid artery for continuous measurements of arterial blood pressure and to allow for blood sampling for blood gas analysis (RAPIDPoint 500, Siemens, Munich, Germany). Arterial blood pressure and heart rate were continuously recorded using PowerLab software (PowerLab 8/35, Chart 8.1.2; AD Instruments Pty, Ltd., Castle Hill, Australia). Rectal temperature was maintained between 36.5 and 37.5 °C using a heating path.

van den Brom C.E., Bozic C., Polet C.A., Bongers A., Tuip-de Boer A.M., Ibelings R., Roelofs J.J, & Juffermans N.P. (2024). Effect of antibody-mediated connective tissue growth factor neutralization on lung edema in ventilator-induced lung injury in rats. Molecular Medicine, 30, 68.

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Detailed Anesthesia Induction and Maintenance Protocol

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Anaesthesia was induced as described previously. 3 Briefly, anaesthesia was induced with isoflurane 5% in oxygen, and when the pedal withdrawal response to a nociceptive stimulus was absent, tracheal intubation with a 16G catheter was performed (Venflon Pro; Becton Dickinson, Helsingborg, Sweden). Mechanical ventilation (UMV-03; UNO Roestvaststaal BV, Zevenaar, The Netherlands; tidal volume 10 ml kg À1 , frequency 60e65 min À1 , PEEP 2e4 cm H 2 O) was initiated and anaesthesia was maintained with isoflurane 2e3% with the FiO 2 at 0.35. The body temperature was maintained at 36.5 C. A 22G catheter (Venflon Pro; Becton Dickinson) was inserted in the tail artery for arterial blood-pressure monitoring. Fentanyl 12 mg kg À1 (Janssen-Cilag, Tilburg, The Netherlands) was then administered, and repeated before the onset of CPB and after 40 min of CPB, and the inhaled isoflurane concentration was reduced to 1.5e2.0%. Further information can be found in the Supplementary File.

Koning N.J., de Lange F., van Meurs M., Jongman R.M., Ahmed Y., Schwarte L.A., van Nieuw Amerongen G.P., Vonk A.B.A., Niessen H.W., Baufreton C, & Boer C. (2018). Reduction of vascular leakage by imatinib is associated with preserved microcirculatory perfusion and reduced renal injury markers in a rat model of cardiopulmonary bypass. British journal of anaesthesia, 120(6).

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Venous Blood Sampling During Exercise

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Venous blood samples were taken three times from an antecubital vein via peripheral venous catheter 1.3 × 32 mm (BD Venflon Pro, Becton Dickinson, Helsingborg, Sweden): (i) before exercise, (ii) immediately after exercise (at exhaustion), and (iii) after 30 min of recovery. Blood samples were taken into two separate tubes with EDTA (2.7 mL) and lithium heparinate (4.9 mL) as anticoagulants (S-monovette, Sarstedt, Nümbrecht, Germany). The first tube was used for the determination of hematocrit (Hct) value and hemoglobin (Hb) concentration. The second tube was used to assess all other compounds: lactate (LA) in whole blood, erythrocyte purine nucleotides (ATP, ADP, AMP, IMP, GTP, guaninediphosphate (GDP), GMP), erythrocyte hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity, plasma adenosine (Ado), inosine (Ino), guanosine (Guo), and hypoxanthine (Hx).

Pospieszna B., Kusy K., Słomińska E.M., Dudzinska W., Ciekot-Sołtysiak M, & Zieliński J. (2019). The Effect of Training on Erythrocyte Energy Status and Plasma Purine Metabolites in Athletes. Metabolites, 10(1), 5.

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Catheterization for Metabolic Studies

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One catheter (BD Venflon, PRO, Becton Dickinson, Singapore) was inserted into an antecubital vein for the infusion of GIP, glucose and insulin. The subjects were then catheterized in a subcutaneous vein on the anterior abdominal wall and in a radial artery.
A vein draining the subcutaneous abdominal adipose tissue on the anterior abdominal wall was catheterized antegradely as previously described^{13 (link)} during ultrasound/color-Doppler imaging of the vein. A 22-g 10-cm polyurethane catheter (Arrow International, Reading, PA, USA) was inserted using the Seldinger technique. The tip of the catheter was positioned above the inguinal ligament in order to minimize the risk of withdrawing blood from the femoral vein. After insertion, the catheter was kept patent throughout the experiment by continuous infusion of saline at a rate of 40 ml h⁻¹. Another catheter was inserted percutaneously into the radial artery of the non-dominant arm under local analgesia (1 ml 1% lidocaine) with an Artflon (Becton Dickinson, Erembodegem, Belgium). The catheter was kept patent by regular flushing with saline.

Asmar M., Arngrim N., Simonsen L., Asmar A., Nordby P., Holst J.J, & Bülow J. (2016). The blunted effect of glucose-dependent insulinotropic polypeptide in subcutaneous abdominal adipose tissue in obese subjects is partly reversed by weight loss. Nutrition & Diabetes, 6(5), e208-.

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Venous Blood Sampling During Exercise

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Blood samples were obtained from the antecubital vein via peripheral venous catheter (1.3× 32 mm, BD Venflon Pro, Becton Dickinson, Helsingborg, Sweden) into syringes comprising EDTA (S-Monovette, 2.7 ml KE; Sarstedt, Nümbrecht, Germany) by the medical staff, at rest, at the end of each 3-min stage above 10 km ⋅ h⁻¹, immediately after exercise and in the 5th, 10th, 15th, 20th, and 30th min of post-exercise recovery.

Ciekot-Sołtysiak M., Kusy K., Podgórski T., Pospieszna B, & Zieliński J. (2024). Changes in red blood cell parameters during incremental exercise in highly trained athletes of different sport specializations. PeerJ, 12, e17040.

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Exercise-Induced Blood Metabolite Dynamics

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Venous blood samples were obtained at rest, at the end of each 3-min stage above 10 km·h⁻¹, immediately after exercise and in the 5th, 10th, 15th, 20th, and 30th min of postexercise recovery. The catheter (1.3 × 32 mm, BD Venflon Pro, Becton Dickinson, Helsingborg, Sweden) was inserted retrogradely into the antecubital vein which was kept patent with isotonic saline (0.9% NaCl) during the whole procedure. Syringes comprising EDTA (S-Monovette, 2.7 ml KE, Sarstedt, Nümbrecht, Germany) were used for hematological parameters and plasma nucleotide concentrations analyses. For lactic acid measurement, lithium heparin as an anticoagulant (S-Monovette, 2.7 ml KE, Sarstedt, Nümbrecht, Germany) was used.

Zarębska E.A., Kusy K., Słomińska E.M., Kruszyna Ł, & Zieliński J. (2018). Plasma Nucleotide Dynamics during Exercise and Recovery in Highly Trained Athletes and Recreationally Active Individuals. BioMed Research International, 2018, 4081802.

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Blood Sample Collection and Processing

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Following RMR assessments, a 20-G cannula (BD Venflon™ Pro, Becton, Dickinson & Co., Sweden) will be inserted into an antecubital vein from which baseline 20 mL of blood will be drawn through two 10-mL syringes and placed into serum separation beads and EDTA-containing tubes (Sarstedt Ltd, Leicester, UK). Plasma samples will be centrifuged immediately at 3466g at 4 °C for 10 min (Heraeus Biofuge Primo R, Kendro Laboratory Products Plc., Tyne and Wear, UK). Serum samples will be left to clot for 15 min at room temperature before centrifugation. All samples will be dispensed into 0.5-mL aliquots and immediately cooled on dry ice and then stored at −80 °C. A small aliquot of EDTA blood will be used to obtain the full leucocyte differential and other haematological variables (SF-300, Sysmex Ltd., Milton Keynes, UK).

Walhin J.P., Chen Y.C., Hengist A., Bilzon J., Betts J.A, & Thompson D. (2018). The effects of different forms of daily exercise on metabolic function following short-term overfeeding and reduced physical activity in healthy young men: study protocol for a randomised controlled trial. Trials, 19, 199.

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Acute Exercise Blood Sampling Protocol

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Blood was collected from the antecubital vein using a peripheral venepuncture (BD Venflon Pro, Becton Dickinson, Helsingborg, Sweden). Samples for gene expression determination were taken into monovette with EDTA (S-monovette K3 EDTA, 7.5 ml, Sarstedt, Nümbrecht, Germany), and samples for lactate concentration were collected in lithium heparin monovette (S-monovette, 2.7 ml KE, Sarstedt, Nümbrecht, Germany). Blood was collected after 3 min of standing on the treadmill (rat rest), every 3 min above the speed of 8 km/h, and after 5, 10, 15, 20, and 30 min of recovery. Table 1 shows the blood sampling scheme.Table 1

Time points (stages) at which blood samples were taken

Collection point	Test stage	Total time (min)
1	At rest (before the test)	0
2	Running ‒ at 10 km/h	12
3	‒ at 12 km/h	15
4	‒ at 14 km/h	18
5	‒ at 16 km/h	21
6	‒ at 18 km/h	24
7	Test termination (exhaustion)	24‒27*
8	Recovery ‒ 5 min	32
9	‒ 10 min	37
10	‒ 15 min	42
11	‒ 20 min	52
12	‒ 30 min	62

*The range results from the variation in particpants’ maximum aerobic capacity

Światowy W.J., Zieliński J., Osielska M.A., Kusy K., Wieliński D., Pławski A, & Jagodziński P.P. (2022). No dynamic changes in the expression of genes related to the epigenetic mechanism during acute exercise. Journal of Applied Genetics, 64(1), 81-87.

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LPS-Induced Lung Injury Model

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Twenty-four hours prior to mechanical ventilation, mild lung injury was induced by LPS administration. After sedation of the rats with 4% isoflurane, lungs were intubated with a 16G catheter (Venflon Pro, Becton Dickinson, Helsingborg, Sweden) and LPS of Escherichia Coli (7.5 mg/kg, Sigma-Aldrich, Saint Louis, Missouri, USA) was inoculated intratracheally in a total volume of 100 µl sterile saline. Subsequently, the tracheal tube was rinsed twice with 150 µl of air and removed. Rats were intensively observed for 24 h.

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