Black walled 96 well imaging microplate

Manufactured by Corning

The Black walled 96-well imaging microplate is a laboratory equipment designed for high-throughput cell-based assays and imaging applications. It features a 96-well format with black walls and a clear bottom, which helps to reduce well-to-well crosstalk and improve image quality.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Image it fixation permeabilization kit, by Thermo Fisher Scientific (2 mentions) Actin green probes, by Thermo Fisher Scientific (2 mentions) Cytation 5 cell imaging, by Agilent Technologies (1 mentions)

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96-well microplates (290 citations) 96 wells (10 citations) Black-walled microplates (2 citations)

2 protocols using black walled 96 well imaging microplate

Quantifying Micronuclei in Cancer Cells

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HCC15 CTNNB1Δ cells expressing vector alone or CTNNB1 variants were plated in a black walled 96-well imaging microplate (Corning, NY) at a density of 1,000 cells/well. Mock and irradiated (6 Gy) cells were fixed and permeabilized 72 hours post treatment using the Image-iT Fixation/Permeabilization Kit (Thermo Fisher Scientific). After fixation, the cytosol and nuclei were stained using actin green probes (green; ThermoFisher, MA) and DAPI (blue; ThermoFisher, MA), respectively. For each cell line, at least 4 wells with 16 images per well (64 images in total) were captured at 40X magnification using a Cytation 5 cell imaging multimode reader (BioTek, VT). All images were processed manually using the ImageJ software. We applied an ellipsoid fit and used size and circularity parameters based on the DAPI intensity to quantify micronuclei (24 ).

Gopal P., Yard B.D., Petty A., Lal J.C., Bera T.K., Hoang T.Q., Buhimschi A.D, & Abazeed M.E. (2022). The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation. Clinical Cancer Research, 28(24), 5343-5358.

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Quantifying Micronuclei in HCC15 Cells

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HCC15 CTNNB1Δ cells expressing vector alone or CTNNB1 variants were plated in a black walled 96-well imaging microplate (Corning, NY) at a density of 1000 cells/well. Mock and irradiated (6 Gy) cells were fixed and permeabilized 72 hours post treatment using the Image-iT Fixation/Permeabilization Kit (Thermo Fisher Scientific). After fixation, the cytosol and nuclei were stained using actin green probes (green; ThermoFisher, MA) and DAPI (blue; ThermoFisher, MA), respectively. For each cell line, at least 4 wells with 16 images per well (64 images in total) were captured at 40X magnification using a Cytation 5 cell imaging multimode reader (BioTek, VT). All images were processed manually using the ImageJ software. We applied an ellipsoid fit and used size and circularity parameters based on the DAPI intensity to quantify micronuclei²⁴.

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