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Rabbit anti gfp and mouse anti βactin

Manufactured by Abcam

Rabbit anti-GFP and mouse anti-βactin are primary antibodies used in various immunodetection techniques, such as western blotting, immunohistochemistry, and immunocytochemistry. The rabbit anti-GFP antibody specifically recognizes the green fluorescent protein (GFP), while the mouse anti-βactin antibody targets the beta-actin protein, which is commonly used as a loading control.

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2 protocols using rabbit anti gfp and mouse anti βactin

1

Generation of Rat and Rabbit Antibodies to VAPB and PTPIP51

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Rat and rabbit antibodies to VAPB and PTPIP51 were generated by immunisation with GST-VAPB(1-220) and GST-PTPIP51(36-470) respectively20 (link). Rabbit anti-PTPIP51 was from Atlas, rabbit anti-hemagglutin (HA), mouse anti-α-tubulin (DM1A) and rabbit anti-Mitofusin2 were from Sigma. Rabbit anti-TOM20 was from Santa Cruz Biotechnology. Rabbit anti-GFP and mouse anti-βactin were from Abcam. Mouse PDI (RL77) was from Affinity Bioreagents. Rabbit anti-TDP-43 (12892-1-AP) was from Proteintech. Antibodies to total and ser9 phosphorylated (inactive) GSK3β were from BD Transduction Labs (mouse 610201) and Cell Signalling (rabbit 9336) respectively. The concentrations used for the different antibodies are shown in Supplementary Table 1. Secondary antibodies were horseradish peroxidase-coupled goat anti-mouse, anti-rabbit and anti-rat Igs (GE Healthcare), and Alexa fluorophore (350, 488 and 568) coupled goat anti-mouse and anti-rabbit IgGs (Invitrogen). AR-A014418 and CT99021 were from Abcam and Cayman respectively.
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2

Antibodies for VAPB and PTPIP51 analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rat and rabbit antibodies to VAPB and PTPIP51 were generated by immunization with
GST-VAPB(1–220) and GST-PTPIP51(36–470),
respectively20 (link). Rabbit anti-PTPIP51 was from Atlas, rabbit
anti-haemagglutin (HA), mouse anti-α-tubulin (DM1A) and rabbit
anti-Mitofusin-2 were
from Sigma. Rabbit anti-TOM20 was from Santa Cruz Biotechnology. Rabbit anti-GFP
and mouse anti-βactin were from Abcam. Mouse PDI (RL77) was from Affinity
Bioreagents. Rabbit anti-TDP-43 (12892-1-AP) was from Proteintech. Antibodies to
total and ser9-phosphorylated (inactive) GSK-3β were from BD Transduction Labs (mouse
610201) and Cell Signalling (rabbit 9336), respectively. The concentrations used
for the different antibodies are shown in Supplementary Table 1. Secondary antibodies were horseradish
peroxidase-coupled goat anti-mouse, anti-rabbit and anti-rat Igs (GE
Healthcare), and Alexa fluorophore (350, 488 and 568)-coupled goat anti-mouse
and anti-rabbit IgGs (Invitrogen). AR-A014418 and CT99021 were from Abcam and Cayman, respectively.
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