Manikya Bhasma was obtained from local Baidyanath store in Guwahati city, acridine orange, propidium iodide, ethidium bromide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), agarose, 2,7-dichlorofluorescein diacetate (DCFH-DA), dulbecco’s modified eagle’s medium were purchased from Sigma Aldrich (St. Louis, MO, USA). RNase A, Proteinase K, DMSO, Foetal Bovine Serum (FBS), Penicillin-Streptomycin (100X) antibiotic solution, Phosphate Buffer Saline (PBS), sodium azide, trypan blue, and trypsin were obtained from HiMedia (Mumbai, India). Ethylenediaminetetraacetic acid (EDTA), ethanol, sodium chloride, was purchased from Merck, Germany. Anti-Cyt-c antibodies, Mitotracker Red, and JC-1 dye were obtained from BD-Biosciences (San Jose, USA). The caspase-9 colorimetric kit was obtained from Invitrogen Corporation (Waltham, USA). All the cell culture plates and dishes were purchased from Corning, Lowell, MA, USA. MDAMB-231, DLD-1, HCT-116, MG-63, HeLa cancer cell lines were procured from National Centre for Cell Sciences, Pune, India. All other reagents and chemicals were of analytical grade purity.
Cell culture dishes and plates
Manufactured by Corning
Sourced in United States
Cell culture dishes and plates are laboratory equipment designed for the growth and maintenance of cells in a controlled environment. They provide a sterile, enclosed surface for culturing cells, allowing researchers to study cellular behavior and perform various experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by HiMedia (1 mentions)
Agarose, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by HiMedia (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Proteinase k, by HiMedia (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Caspase 9 colorimetric kit, by Thermo Fisher Scientific (1 mentions)
Jc 1 dye, by BD (1 mentions)
Mitotracker red, by BD (1 mentions)
Trypan blue, by HiMedia (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Sodium azide, by HiMedia (1 mentions)
Trypsin, by HiMedia (1 mentions)
Dimethyl sulfoxide (dmso), by HiMedia (1 mentions)
Rnase a, by HiMedia (1 mentions)
Pregnant mare serum gonadotropin, by Merck Group (1 mentions)
5 protocols using cell culture dishes and plates
1
Assessment of Manikya Bhasma's Anticancer Potential
2
Standardized Cell Culture Protocol for Ovarian Cell Studies
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DMEM: F-12, fetal bovine serum (FBS) and all other cell culture solutions were procured from Invitrogen (Carlsbad, CA, United States). TOP5300 and TOP5668 were synthesized at Chempartner (Shanghai, China) as part of the hit-to-lead program managed by TocopheRx. Structure and purity of the compounds were confirmed by NMR, LC/MS and HPLC. The purity of the compound employed for the study ranged from 95–97%. Recombinant human FSH (rec-hFSH) and human chorionic gonadotropin (hCG) were bulk preparations of Merck KGaA (follitropin-α, choriogonadotropin-α, Darmstadt, Germany). Pregnant mare serum gonadotropin (PMSG) and other fine chemicals were obtained from Sigma-Aldrich (St; Louis, MO, United States). Cell culture plates and dishes were purchased from Corning Inc (Corning, NY, United States). Diethylstilbesterol (DES) time release pellets and trochar were obtained from Innovative Research of America (Sarasota, FL, United States).
3
Nanoparticle-based Immunotherapy Protocol
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Gadolinium acetate hydrate and skeletal muscle Mb were purchased from Sigma–Aldrich (USA). Triethylamine was purchased from Alfa Aesar (USA). Distearoylphosphatidylcholine (DSPC) and DSPC-polyethylene glycol 2000 (DSPE-mPEG2000) were purchased from Avanti Polar Lipids (USA). Cholesterol was purchased from Tokyo Chemical Industry (Japan). Cell culture dishes and plates were purchased from Corning (USA). Mouse IFN-γ ELISA Kit (Catalog No. CZM10-96) was purchased from Beijing CHENG ZHI KE WEI Biotechnology Co., Ltd. (China). Mouse IL-1β ELISA Kit (Catalog No. EMC001bQT) was purchased from NeoBioscience Technology Co., Ltd. (China). Ultrapure water was obtained using a Millipore Milli-Q Gradient System (USA). Rabbit anti-γ-H2A.X (phospho S139) antibody (Catalog No. ab81299, Clone: EP854(2)Y, 1: 250) was purchased from Abcam (USA). FITC-conjugated goat anti-rabbit IgG H&L antibody (Catalog No. ab6717, 1: 1000) was purchased from Abcam (USA). APC anti-mouse CD3 antibody (Catalog No. 100312, Clone: 145-2C11, 1: 100), FITC anti-mouse CD8 antibody (Catalog No. 100706, Clone: 53-6.7, 1: 100), PE/Cyanine7 anti-mouse IFN-γ antibody (Catalog No. 505826, Clone: XMG1.2, 1: 100), PE anti-mouse/human CD44 antibody (Catalog No. 103008, Clone: IM7, 1: 100), and PE/Cyanine7 anti-mouse CD62L antibody (Catalog No. 104418, Clone: MEL-14, 1: 100) were purchased from BioLegend (USA).
4
Alginate-based Hydrogel for Cell Culture
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Pharmaceutical-grade sodium alginate (Keltone® LV) was a gift from FMC BioPolymer Corporation (Philadelphia, PA, USA). Calcium chloride, dexamethasone, beta-glycerophosphate, 2-phospho-L-ascorbic acid and citric acid were procured from Sigma-Aldrich (St. Louis, MO, USA).
Cell culture medium, antibiotics, fetal bovine serum, trypsin-EDTA and TRIzol® were obtained from Life Technologies, Inc. (Carlsbad, CA, USA). Cell culture dishes and plates were obtained from Corning Life Sciences, Inc. (Acton, MA, USA). OmicsGreen qPCR Master Mix was sourced from Omics Biotechnology Co. (Taipei, Taiwan). Optical reaction plate, and optical adhesive covers for real-time PCR were procured from Life Technologies, Inc. (Carlsbad, CA, USA).
Cell culture medium, antibiotics, fetal bovine serum, trypsin-EDTA and TRIzol® were obtained from Life Technologies, Inc. (Carlsbad, CA, USA). Cell culture dishes and plates were obtained from Corning Life Sciences, Inc. (Acton, MA, USA). OmicsGreen qPCR Master Mix was sourced from Omics Biotechnology Co. (Taipei, Taiwan). Optical reaction plate, and optical adhesive covers for real-time PCR were procured from Life Technologies, Inc. (Carlsbad, CA, USA).
5
Bovine Aortic Endothelial Cell Culture Protocol
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Bovine aortic endothelial cells were purchased from Cell Applications (San Diego, CA, USA). The following materials were purchased from the indicated vendors: Dulbecco's modified Eagle's medium and calcium-and magnesium-free phosphate buffered saline from Nissui Pharmaceutical (Tokyo, Japan); fetal bovine serum, Opti-MEM ® Reduced Serum Medium, Lipofectamine ® RNAiMAX Transfection Reagent, and High-Capacity cDNA Reverse Transcription Kit from Thermo Fisher Scientific (Waltham, MA, USA); cell culture dishes and plates from Corning (Corning, NY, USA); QIAzol lysis reagent from QIAGEN (Venlo, The Netherlands); GeneAce SYBR ® qPCR Mix α from Nippon Gene (Tokyo, Japan); and 1,3-diaminopropane from Tokyo Chemical Industry (Tokyo, Japan). Other reagents were purchased from Nacalai Tesque (Kyoto, Japan).
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