HL-1 cells grown on cover slips were fixed with 4% paraformaldehyde for 15 min. After being washed with PBS, the cells were permeabilized by incubation with 0.5% Triton X-100/PBS for 5 min, and washed with PBS. For myofibril staining, the cells were incubated with 10 μg/ml Phalloidin-Tetramethylrhodamine B isothiocyanate (#P1951, Sigma Aldrich) in PBS for 10 min at RT. For YAP staining, the cells were incubated with anti-total-YAP antibody overnight at 4°C, then with Alexa488-conjugated anti-IgG antibody (Molecular probes, USA), and observed under a confocal fluorescence microscope (Nikon Eclipse Ti, Nikon, Tokyo, Japan) or a conventional fluorescence microscope (BZ-8100, Keyence).
Alexa488 conjugated anti igg antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Alexa488-conjugated anti-IgG antibody is a laboratory reagent consisting of an antibody specific to immunoglobulin G (IgG) that has been labeled with the fluorescent dye Alexa Fluor 488. This product can be used for various immunoassay and cell imaging applications that require the detection of IgG molecules.
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Lab products found in correlation
2 protocols using alexa488 conjugated anti igg antibody
1
Immunofluorescence Staining of HL-1 Cells
2
Immunostaining of YAP and Actin Filaments
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The cells were fixed with 4% paraformaldehyde for 15 min., and permeabilized with 0.5% Triton X-100 for 5 min. For immunocytochemical examination of YAP, the cells were incubated with anti-YAP antibody overnight at 4°C. Then, the cells were incubated with Alexa488-conjugated anti-IgG antibody (Molecular Probes, Eugene, OR, USA), and observed under a fluorescence microscope (Leica, DMi8, Wetzlar, Germany). Nuclei were also counterstained with DAPI. For staining actin filaments, the cells were incubated with Phalloidin-Tetramethylrhodamine B (10 μg/mL, Sigma-Aldrich) for 10 min, and observed under a confocal fluorescence microscope (Eclipse Ti, Nikon, Tokyo, Japan)
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