Monoclonal anti phospho erk1 2 thr202 tyr204

Polyclonal anti-Sprouty2 antibody was obtained from Sigma. The monoclonal anti-phospho-ERK1/2 (Thr202/Tyr204) and polyclonal anti-phospho-CREB, anti-CREB, anti-ERK1/2, anti-phospho-AKT (Ser473) and anti-AKT antibodies were obtained from Cell Signaling (Danvers, MA). The polyclonal anti-COX-2 antibody was obtained from Abcam (Cambridge, MA). The monoclonal anti-α-tubulin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG were obtained from Bio-Rad Laboratories (Hercules, CA). Human chorionic gonadotropin (hCG), 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) and LY294002 were obtained from Sigma. Recombinant human amphiregulin was obtained from R&D systems (Minneapolis, MN). Wortmannin, PD98059 and U0126 were obtained from Calbiochem (San Diego, CA).

Polyclonal anti-StAR antibody was obtained from Santa Cruz Biotechnology (Shanghai, China). Monoclonal anti-α-tubulin antibody was obtained from CMCTAG (Shanghai, China). Monoclonal anti-phospho-ERK1/2 (Thr²⁰²/Tyr²⁰⁴) and polyclonal anti-ERK1/2, anti-phospho-AKT (Ser⁴⁷³), and anti-AKT antibodies were obtained from Cell Signaling Technology (Shanghai, China). Polyclonal anti-MT1 was obtained from Bioworld Technology (Nanjing, China). Polyclonal anti-MT2 was obtained from Abcam (Shanghai, China). Horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse IgGs were obtained from Bio-Rad Laboratories (Shanghai, China). Melatonin, 4-P-PDOT, luzindole, and LY294002 were obtained from Sigma-Aldrich Corp (Shanghai, China).