Cobalt 2 bromide

Tannic acid (TA, ACS reagent), titanium (IV) bis (ammonium lactato) dihydroxide (Ti-BALDH) solutions 50 wt% in H₂O, iron (III) hexahydrate (FeCl₃.6H₂O), copper (II) chloride (CuCl₂), cobalt (II) bromide (CoBr₂), nickel (II) chloride (NiCl₂), zinc (II) chloride (ZnCl₂), 2,6-dichlorophenolindophenol, trizma hydrochloride (Tris), copper assay kit and peroxidase from horseradish (HRP) lyophilized powder were purchased from Sigma-Aldrich. Phosphate buffer saline (PBS) was obtained from Acros Organics. Luria-Bertani (LB) and tryptic soy broth (TSB) were purchased from Gibco. MTT assay kit and sodium hydroxide (NaOH) were acquired from Thermo Fisher Scientific. Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco. NIH 3T3 fibroblasts were obtained from Bioresource Collections and Research Center (BCRC), Taiwan. S. epidermidis ATCC12228, methicillin-resistant S. aureus (USA300, ATCC BAA-1718) and E. coli ATCC12435 were acquired from Bioresource Collections and Research Center in Taiwan. All the water used was purified to 18.2 mΩ using a Millipore water purifications system, and filtered using a 0.22 µm filter.

N,N′-Dimethylpropyleneurea (BASF) was distilled under reduced pressure over calcium hydride, CaH₂ (Sigma-Aldrich), and stored in dark bottles over 3 Å molecular sieves. Anhydrous magnesium, calcium, scandium(iii) and praseodymium(iii) iodide, and cobalt(ii) bromide (Sigma-Aldrich, 99.9% purity) were received in glass ampoules and used as purchased. Anhydrous calcium trifluoromethanesulfonate, Ca(CF₃SO₃)₂, was prepared by dissolving calcium oxide (Merck, 99% purity) in dilute trifluoromethanesulfonic acid (Fluka), followed by filtration, boiling off excess water and acid, and finally drying and storing in an oven at 470 K. Anhydrous strontium, barium and scandium(iii) trifluoromethanesulfonate were prepared accordingly using strontium, barium and scandium oxide (Merck, 99.9% purity). Hexakis(N,N′-dimethylpropyleneurea)calcium perchlorate, [Ca(dmpu)₆](ClO₄)₂, was prepared by dissolving calcium perchlorate tetrahydrate, Ca(ClO₄)₂·4H₂O (99%, Merck), in a minimum amount of dry acetone and a four-fold excess of 2,2-dimethoxymethane, (CH₃)₂C(OCH₃)₂ (Merck), was added and the mixture was stirred vigorously for two hours.^{15 (link)} A six-fold excess of dmpu was added and the resulting mixture was stirred for another 30 min. White crystals were obtained on cooling.