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2 protocols using ethyl hexanoate

1

Volatile Organic Compounds for Behavior Tests

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The compounds used in behaviour tests namely 3-methylbut-1-yl propionate (≥99% chemical purity), 3-methylbutan-1-ol (≥99% chemical purity), ethyl octanoate (≥99% chemical purity), 2-phenylethyl acetate (≥99% chemical purity) and 2-phenyl ethanol (≥99% chemical purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA); ethyl propionate (99% chemical purity), 3-methylbut-1-yl acetate (99% chemical purity), ethyl hexanoate (99% chemical purity) were purchased from Alfa Aesar (Ward Hill, MA, USA); ethyl acetate (≥99% chemical purity), hexane as a solvent (≥99% chemical purity) was obtained from Carl Roth (Karsruhe, Germany).
D (+)-Glucose monohydrate (obtained from Merck Millipore, Darmstadt, Germany), yeast extract, peptone and agar (purchased from Liofilchem, Rosetodegli Abruzzi, Italy) were used for YPD-agar medium preparation and yeast cultivation. To avoid bacterial contamination, YPD medium was supplemented with chloramphenicol (purchased from Applichem, Darmstadt, Germany).
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2

Analysis of Volatile Organic Compounds

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The following compounds were purchased from Sigma–Aldrich (St. Louis, MO, USA): hexanal (98% chemical purity), (E)-2-hexenal (98% chemical purity), octanal (99% chemical purity), nonanal (95% chemical purity), decanal (≥98% chemical purity), (E)-2-nonenal (97% chemical purity), benzaldehyde (≥99% chemical purity), 6-methyl-5-hepten-2-one (99% chemical purity), 1-hexanol (≥99% chemical purity), (Z)-3-hexen-1-ol (98% chemical purity), 1-octen-3-ol (98% chemical purity), propyl 3-methylbutanoate (≥98% chemical purity), 3-methylbutyl propionate (≥98% chemical purity), 3-methylbutyl 3-methylbutanoate (≥98% chemical purity), hexyl acetate (99% chemical purity), ethyl octanoate (≥99% chemical purity), (Z)-3-hexenyl acetate, (Z)-3-hexenyl 3-methylbutanoate (≥97% chemical purity), α-pinene (98% chemical purity), α-phellandrene (≥95% chemical purity), limonene (≥98% chemical purity), (E)-β-ocimene (≥90% chemical purity), p-cymene (99% chemical purity), linalool (97% chemical purity), α-terpinyl acetate, 2-phenylethyl acetate (99% chemical purity), (E)-geranyl acetone (96% chemical purity), hexane (≥99% chemical purity). Ethyl hexanoate (99% chemical purity), 3-methylbutyl acetate (98% chemical purity) were purchased from Alfa Aesar (Ward Hill, MA, USA), δ-3-cerene from Carl Roth (Karlsruhe, Germany), α-farnesene from Bedoukian Research Inc. (Danbury, CT, USA).
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