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2 protocols using akt rabbit antibody

1

Investigating Cell Signaling Pathways

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Cell culture medium was obtained from Invitrogen (Carlsbad, CA) and fetal bovine serum from Hyclone Thermo (Waltham, MA. #SH300070.03). Antibodies for β-catenin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). LC-3 antibody (NB100-2220) was purchased from Novus biological (Littleton, CO).
GAPDH rabbit mAb (#2118), caspase-3 rabbit mAb (#9665), p-GSK3β (Ser9) rabbit antibody (#5558), GSK3β rabbit antibody (#9315), p-AKT (Thr308) rabbit antibody (#9275), p-AKT (Ser473) rabbit antibody (#4060), AKT rabbit antibody (#9272), Cleaved caspase 3 rabbit antibody (#9664) and caspase 3 rabbit antibody (#9662) were all obtained from Cell Signaling (Beverly, MA).
nCDase antibody (Ab174) was a kind gift from Dr. Rick Proia (Genetics of Development and Disease, NIDDK, Bethesda, MD) 11 (link). The enhanced chemiluminescence kit (ECL) was from ThermoScientific (Rockford, IL). C6 urea-ceramide was provided by the Lipidomics Core facility at MUSC.
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2

Investigating Cell Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture medium was obtained from Invitrogen (Carlsbad, CA) and fetal bovine serum from Hyclone Thermo (Waltham, MA. #SH300070.03). Antibodies for β-catenin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). LC-3 antibody (NB100-2220) was purchased from Novus biological (Littleton, CO).
GAPDH rabbit mAb (#2118), caspase-3 rabbit mAb (#9665), p-GSK3β (Ser9) rabbit antibody (#5558), GSK3β rabbit antibody (#9315), p-AKT (Thr308) rabbit antibody (#9275), p-AKT (Ser473) rabbit antibody (#4060), AKT rabbit antibody (#9272), Cleaved caspase 3 rabbit antibody (#9664) and caspase 3 rabbit antibody (#9662) were all obtained from Cell Signaling (Beverly, MA).
nCDase antibody (Ab174) was a kind gift from Dr. Rick Proia (Genetics of Development and Disease, NIDDK, Bethesda, MD) 11 (link). The enhanced chemiluminescence kit (ECL) was from ThermoScientific (Rockford, IL). C6 urea-ceramide was provided by the Lipidomics Core facility at MUSC.
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