Glycerol

The prototype UMP-Glo assay kit was a generous gift from Promega. HEPES (99.9%) was obtained from Chem-Impex International. NaCl (>99%) and glycerol (>99%) were from Research Products International. Triton X-100 and DMSO (99.9%) were from Sigma-Aldrich, MgCl₂.6H₂O (100%) was from Mallinckrodt Chemicals. DDM (>99%) was purchased from Anatrace. 96-well half-area white plates were obtained from Corning. Ni-NTA resin was from Thermo Fisher. All other chemicals and bio-reagents were purchased at the purest grade available.

All organic solvents (methanol, ethanol, acetone, dimethyl formamide (DMF) and, N,N-diisopropylethylamine (DIPEA)) were HPLC grade (Fisher Scientific) and used without further purification. E. coli cells (BL21 and NEB 5-alpha) were purchased from New England Biolabs. Lysozyme and Sephadex LH-20 were purchased from Sigma-Aldrich. BLUEStain protein ladder and ampicillin were purchased from Gold Biotechnology. Yeast extract, L-proline, tryptone, glycerol, and other bacterial culture media components were purchased from Research Products International. All materials for SDS-PAGE were obtained from Bio-Rad. PE/Cyanine7 anti-human EGFR antibody was purchased from BioLegend. Cy5.5 NHS ester was obtained from Lumi Probe. DMEM/F12, DMEM, fetal bovine serum (FBS), PenStrep, and other cell culture reagents were obtained from Corning Life Sciences.