After 14 days of culture in α-MEM, 10% FBS, and 1% Pen/Strep (all Life Technologies) at 37°C, Ocy454 cells were treated with TPTD 5 nM, ABL 5 nM, or vehicle for 5 minutes to 3 hours. The Ocy454 cells were obtained from Paola Divieti Pajevic from the MGH Center for Skeletal Research, Boston, Massachusetts, USA. Total proteins (10 μg) were resolved by SDS-PAGE under reducing conditions. Immunodetection was performed with S246 phospho-HDAC4/ S259 phospho-HDAC5 (Cell Signaling Technology 3443), phospho-CRCT2 (Abcam, ab203187), HDAC4 (Abcam, ab12172), HDAC5 (Abcam, ab55403), or GAPDH (Santa Cruz Biotechnology, SC-32233). GAPDH was used as loading control. Immunoreactive proteins were detected using SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, 34075). Films were scanned (Canon, CanoScan 9000F) and saved as 600 dpi color images.
S246 phospho hdac4 s259 phospho hdac5
Manufactured by Cell Signaling Technology
The S246 phospho-HDAC4/S259 phospho-HDAC5 product is a research-use antibody that detects the phosphorylation of HDAC4 at serine 246 and HDAC5 at serine 259. These phosphorylation sites play a role in the regulation of histone deacetylase activity and subcellular localization.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using s246 phospho hdac4 s259 phospho hdac5
1
TPTD and ABL Regulate HDAC4/5
2
TPTD and ABL Regulate HDAC4/5
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After 14 days of culture in α-MEM, 10% FBS, and 1% Pen/Strep (all Life Technologies) at 37°C, Ocy454 cells were treated with TPTD 5 nM, ABL 5 nM, or vehicle for 5 minutes to 3 hours. The Ocy454 cells were obtained from Paola Divieti Pajevic from the MGH Center for Skeletal Research, Boston, Massachusetts, USA. Total proteins (10 μg) were resolved by SDS-PAGE under reducing conditions. Immunodetection was performed with S246 phospho-HDAC4/ S259 phospho-HDAC5 (Cell Signaling Technology 3443), phospho-CRCT2 (Abcam, ab203187), HDAC4 (Abcam, ab12172), HDAC5 (Abcam, ab55403), or GAPDH (Santa Cruz Biotechnology, SC-32233). GAPDH was used as loading control. Immunoreactive proteins were detected using SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, 34075). Films were scanned (Canon, CanoScan 9000F) and saved as 600 dpi color images.
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