Melanoma cell lines SK-Mel-19, −28, −29, −94, −103, −147, −173, G-361 were obtained from Memorial Sloan Kettering Cancer Center and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2mM glutamine, and 1% penicillin-streptomycin antibiotics. Populations of normal human melanocytes (NHM) were purchased from Invitrogen and maintained in Medium 254 (Invitrogen) supplemented with human melanocyte growth supplement (Invitrogen). Colo679, UACC-257, WM1366, SCOV3 and HeLa were purchased from ATCC. HMLE and HMLER cells were a gift from Dr. Robert A Weinberg (Whitehead Institute). Melan-a mouse melanocytes were grown at 37°C (10% CO2) in RPMI media containing 12-O-Tetradecanoylphorbol-13-acetate (TPA).
Colo679
Manufactured by American Type Culture Collection
Sourced in United States
The Colo679 is a laboratory equipment that serves as a cell culture vessel. It is designed to support the growth and maintenance of various cell types in a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
Human melanocyte growth supplement, by Thermo Fisher Scientific (1 mentions)
Medium 254, by Thermo Fisher Scientific (1 mentions)
Wm1366, by American Type Culture Collection (1 mentions)
Scov 3, by American Type Culture Collection (1 mentions)
Sk mel 2, by American Type Culture Collection (1 mentions)
Atcc crl 3216, by American Type Culture Collection (1 mentions)
Rpmi 7951, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
4 protocols using colo679
1
Culturing Diverse Melanoma Cell Lines
2
Cell Line Culture Conditions
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293 T, RPMI7951, CJM, A375, SKMEL2, COLO679, and G361 cell lines were purchased from commercial sources (ATCC CRL-3216, ATCC HTB-66, Creative Bioarray CSC-C6421J, ATCC CRL-1619, ATCC HTB-68, Sigma 87061210, and ATCC CRL-1424, respectively) and cultured in DMEM (Life Technologies), except for G361 (McCoy’s media, 10% FBS (Atlanta Biologicals), 1X GlutaMAX (Life Technologies) and 1% penicillin-streptomycin (Life Technologies)). Cells were grown at 37 °C in 5% CO2. All cultures were regularly checked for the presence of mycoplasma. For cell culture experiments implicating genetic or pharmacological modifications, cells were randomly distributed between the different experimental conditions.
3
Culturing Human Melanoma and Keratinocyte Cell Lines
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The human malignant melanoma (A375) and (COLO-679) cell lines were purchased from the American Type Culture Collection (ATCC) Manassas, VA, USA and Deutsche Sammlung von Microorganismen und Zellkulturen (DSMZ) Braunschweig, Germany, respectively. The human immortalized keratinocyte (HaCaT) cell line was kindly provided by Dr Sharon Broby (Dermal Toxicology and Effects Group; Centre for Radiation, Chemical and Environmental Hazards; Public Health England, Chilton, UK). A375 cells were cultured in DMEM high glucose whereas COLO-679 cells were grown in RPMI media. Both types of culture media were supplemented with 10% FBS, 2 mM L-glutamine and 1% pen/strep (100 U/mL penicillin/100 μg/mL streptomycin). Cells were grown in a humidified incubator at 37 °C and 5% CO2, as monolayers and sub-cultured at 80–90% confluency.
4
Cell Culture of Melanoma and Keratinocyte Lines
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The human malignant melanoma cell lines (A375 and COLO-679) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and Deutsche Sammlung von Microorganismen und Zellkulturen (DSMZ-Braunschweig, Germany), respectively. The human immortalized keratinocyte (HaCaT) cell line was kindly provided by Dr. Sharon Broby (Dermal Toxicology & Effects Group; Centre for Radiation, Chemical, and Environmental Hazards; Public Health England, Didcot, UK). A375 and HaCaT cells were cultured in DMEM (high glucose), whereas COLO-679 cells were grown in RPMI media. All media were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 1% pen/strep (100 U/mL penicillin, 100 μg/mL streptomycin). Cells were cultured in a humidified incubator at 37 °C and 5%CO2, grown as monolayers, and sub-cultured at 80–90% confluency. All cell lines were cultured for 15–20 passages before new stocks were utilized.
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