For RNA isolation from cell cultures, the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) was used. RNA isolation from biopsies was done with TRI Reagent® (Sigma-Aldrich, Taufkirchen, Germany), followed by DNase (NEB, Frankfurt a.M., Germany) treatment. CDNAs were synthesized from 250 ng to 1000 ng of total RNA using the RevertAid RT synthesis kit (Thermo Scientific, Darmstadt, Germany) according to the manufacturer’s protocol.
Revertaid rt synthesis kit
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The RevertAid RT synthesis kit is a reagent system designed for the reverse transcription of RNA to complementary DNA (cDNA). The kit includes all the necessary components for the reverse transcription reaction, including the RevertAid Reverse Transcriptase enzyme, reaction buffer, and dNTPs. The kit is suitable for use with a variety of RNA templates, including mRNA, total RNA, and viral RNA.
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Lab products found in correlation
3 protocols using revertaid rt synthesis kit
1
RNA Isolation and cDNA Synthesis
2
Total RNA Extraction and cDNA Synthesis
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Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. cDNAs were synthesized from 500 ng of total RNA using the RevertAid RT synthesis kit (Thermo Scientific, Darmstadt, Germany).
3
RNA Isolation and Reverse Transcription
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Total RNA from 20 µg head kidney sample was isolated using RNeasy Plus Micro RNA isolation kit (Qiagen, Germany) according to manufacturer's protocol. Total RNA was treated with 1 U DNase I (Thermo, Lithuania) and reverse transcribed using Revert Aid RT synthesis kit (Thermo, Lithuania), making 1 µg of template RNA, 15 pmol/µL oligo dT primer, 0 U/µL Revert Aid M-MuLV RT enzyme, 1 mM of dNTP mix, 4 µL 5× reaction buffer and 6 µL of NFW. cDNA was synthesised using thermal cycler (Thermo, Lithuania) for 60 min at 42°C.
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