The BEAS-2B cell line, derived from human bronchial epithelial cells transformed by an adenovirus 12-SV40 hybrid virus, was purchased from the European Collection of Cell Cultures (Salisbury, Wiltshire, UK). Airway epithelial cells were seeded in collagen-I-coated 96- or 12-well plates and incubated for 72 h to reach semiconfluence. The cells were incubated in serum-free medium LHC-9 (Life Technologies, Carlsbad, CA, USA) at 37 °C in a humidified atmosphere containing 5% CO2.
Beas 2b cell line
Manufactured by European Collection of Cell Cultures
Sourced in United Kingdom
The BEAS-2B cell line is a human bronchial epithelial cell line derived from normal human bronchial epithelial cells. The cells are used for in vitro studies of respiratory biology and disease.
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5 protocols using beas 2b cell line
1
Culturing Adenovirus-transformed Human Bronchial Epithelial Cells
2
Culturing Human Bronchial Epithelial Cells
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The BEAS-2B cell line, derived from human bronchial epithelial cells transformed by an adenovirus 12-SV40 hybrid virus, was purchased from European Collection of Cell Cultures (Salisbury, Wiltshire, UK). Airway epithelial cells were seeded in 96-well or 12-well collagen I-coated plates and incubated for 72 h to reach semi-confluence in the serum-free medium LHC-9 (Life Technologies, Carlsbad, CA, USA) at 37°C in a humidified atmosphere of 5% CO2.
3
Culturing BEAS-2B Bronchial Epithelial Cells
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The BEAS-2B cell line, derived from human bronchial epithelial cells transformed by an adenovirus 12-SV40 hybrid virus, was purchased from the European Collection of Cell Cultures (Salisbury, Wiltshire, UK). The cells (7.5×10 4 cells/mL) were seeded in 96-well (0.07 mL) or 12-well (0.7 mL) collagen I-coated plates and incubated for 72 h to reach semiconfluence in the serum free-medium LHC-9 (Life Technologies) at 37°C in a humidified atmosphere of 5% CO2.
4
Culturing BEAS-2B Bronchial Epithelial Cells
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The BEAS-2B cell line, which is derived from human bronchial epithelial cells transformed by an adenovirus (12-SV40 hybrid virus) was purchased from the European Collection of Cell Cultures (Salisbury, Wiltshire, UK).
To initiate the cell culture, the vial containing the cells was taken out from liquid nitrogen and added into serum-free LHC-9 medium (Life Technologies, Carlsbad, CA, USA). The subculture was maintained in LHC-9 medium in an incubator in a 5% CO2 atmosphere at 37°C.
For particular experiments, cells were seeded in 96-and 12-well collagen I-coated plates and incubated for 72 hr to reach semi-confluence in LHC-9 medium with the same conditions as those used for the subculture.
To initiate the cell culture, the vial containing the cells was taken out from liquid nitrogen and added into serum-free LHC-9 medium (Life Technologies, Carlsbad, CA, USA). The subculture was maintained in LHC-9 medium in an incubator in a 5% CO2 atmosphere at 37°C.
For particular experiments, cells were seeded in 96-and 12-well collagen I-coated plates and incubated for 72 hr to reach semi-confluence in LHC-9 medium with the same conditions as those used for the subculture.
5
BEAS-2B Cell Line Culturing Protocol
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The BEAS-2B cell line (ECCAC no. 95102433) was purchased from the European Collection of Cell Cultures (Salisbury, UK). Cultures were established in tissue culture vessels containing LHC-9 medium (ca 0.2 mL/cm 2 of growth surface) and were maintained at 37 °C, in a humidified atmosphere of 95% air/5% CO 2 . All tissue culture vessels were pre-coated with a mixture of gelatin (type B) and bovine serum albumin (BSA).
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