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2 protocols using methylene violet

1

Evaluation of Heavy Metal Adsorption

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Bisphenol S, bisphenol A, methylene violet, methylene blue, 1-octanesulphonic acid sodium salt, graphite flakes, sodium nitrate (99.0%), hydrochloric acid, sulphuric acid (35%), potassium permanganate (99.0%), hydrogen peroxide (35%), methanol (99.9%), cadmium nitrate, sodium arsenate, copper nitrate, lead nitrate, nickel sulphate, cobalt chloride, mercury(ii) acetate, zinc nitrate and lithium chloride were purchased from Sigma-Aldrich, USA. Analytical grade reagents were used throughout the entire study. De-ionized water was used for all studies.
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2

Characterization of Fluorescent Probes

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6-amino-2-naphthoic acid (ANA), 4-aminobenzoic acid (ABA), 4′-amino-[1,1′-biphenyl]-4-carboxylic acid (ABPA), methylene violet (Bernthsen) (MV), coumarin 343 (C343), β-cyclodextrin (β-CD), cucurbit[7]uril (CB7), citric acid/sodium citrate, sodium dihydrogen phosphate/disodium hydrogen phosphate, boric acid/sodium borate and sodium N-cyclohexyl-3-aminopropanesulfonate (CAPS) were obtained from Sigma Aldrich UK and were of the highest purity available.
5, 6-diamino-naphthalene-1, 3-disulfonic acid (DANDSA) was obtained from Molekula, UK.
4-pyridinium phenolate (4PP) was synthesised and provided by WuXi App Tec (Hong Kong) Ltd. Structure and purity were confirmed by NMR.
The provenances of Brooker's merocyanine (BM), bis-dimethylaminofuchsone (BDF), phenol blue (PB), and 2, 6-dichloro-4-(2, 4, 6-triphenyl-pyridinium-1-yl)-phenolate (ET33) are as stated previously.
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ROW was used throughout this study and had a resistivity of 15MΩcm (checked daily).
Disposable high purity UV-transparent cuvettes (Brand GmbH) with stoppers were used throughout and are described previously.
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Disposable four-sided optically transparent fluorescence cuvettes (Brand GmbH) made of the same material as the UV cuvettes, with stoppers, were used to record fluorescence spectra.
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