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Plenti ifi204 myc ddk puro

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The PLenti-Ifi204-Myc-DDK-Puro is a lentiviral expression vector that allows for the expression of the Ifi204 gene with a Myc and DDK tag, as well as puromycin resistance. This vector can be used for the stable transduction of mammalian cells.

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Lab products found in correlation

Plenti c mgfp, by OriGene (2 mentions) Lenticrisprv2 blast plasmid, by Addgene (2 mentions) N174 mcs, by Addgene (2 mentions) Plenti cd1d1 mgfp, by OriGene (2 mentions) Fastdigest protocol, by Thermo Fisher Scientific (2 mentions) Nebuilder hifi dna assembly cloning kit, by New England Biolabs (2 mentions)

2 protocols using plenti ifi204 myc ddk puro

1

Lentiviral CD1d1 and IFI204 constructs

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A lentiviral murine Cd1d1 expression construct (pLenti-Cd1d1-mGFP, Cat#MR226027L4) and its matched control construct (pLenti-C-mGFP, Cat#PS100093) were obtained from Origene. Murine Ifi204 expression vector (pLenti-Ifi204-Myc-DDK-Puro, Cat#MR222527L3), together with its control vector (pLenti-C-Myc-DDK-Puro, Cat#PS100092) were also purchased from Origene, and the puromycin selection cassette of these two Origene plasmids were replaced by blasticidin from lentiCRISPRv2-blast plasmid (Addgene#98293) using NEBuilder HiFi DNA Assembly Cloning kit (NEB, Cat#E5520S). For the LLC-1 experiment, the murine Cd1d1 was cloned into the receiving vector N174-MCS (Addgene#81061) with the restriction enzymes EcoR1 and Mlu1, using the FastDigest protocol of Thermo Scientific. All final construct sequences were confirmed by Sanger sequencing. Plasmids generated in this study are available upon written request.
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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2

Lentiviral CD1d1 and IFI204 constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols
A lentiviral murine Cd1d1 expression construct (pLenti-Cd1d1-mGFP, Cat#MR226027L4) and its matched control construct (pLenti-C-mGFP, Cat#PS100093) were obtained from Origene. Murine Ifi204 expression vector (pLenti-Ifi204-Myc-DDK-Puro, Cat#MR222527L3), together with its control vector (pLenti-C-Myc-DDK-Puro, Cat#PS100092) were also purchased from Origene, and the puromycin selection cassette of these two Origene plasmids were replaced by blasticidin from lentiCRISPRv2-blast plasmid (Addgene#98293) using NEBuilder HiFi DNA Assembly Cloning kit (NEB, Cat#E5520S). For the LLC-1 experiment, the murine Cd1d1 was cloned into the receiving vector N174-MCS (Addgene#81061) with the restriction enzymes EcoR1 and Mlu1, using the FastDigest protocol of Thermo Scientific. All final construct sequences were confirmed by Sanger sequencing. Plasmids generated in this study are available upon written request.
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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