For analysis of surface markers, single-cell suspensions were stained with following fluorochrome-conjugated antibodies following established guidelines (61 (link)): FITC-conjugated anti-CD45.1 (Biolegend, 110705), PerCP-Cyanine5.5-conjugated anti-CD8 (Biolegend, 110733), Pacific Blue-conjugated anti-CD8 (Biolegend, 100725), PE-conjugated anti-CD69 (Biolegend, 104508), FITC-conjugated TIM3 (Genetex, GTX54055), APC-conjugated anti-LAG3 (Biolegend, 125210), APC-conjugated anti-PD-1 (Biolegend, 135210), APC-conjugated anti-IL-2 (Biolegend, 503809), APC-conjugated anti-CD25 (Biolegend, 101909), FITC-conjugated anti-IFN-γ (Biolegend, 505805), APC-conjugated anti-TNF-α (Biolegend, 506307), PE-conjugated anti-H-2 K(b)/SIINFEKL Tetramer (Helixgen, HG08T14028), BV421-conjugated KLRG1 (Biolegend, 138413), BV605-CD127 (Biolegend, 135025), and APC-Cy7-conjugated anti-CD62L (Biolegend, 104427), For intracellular staining, cells were first fixed with IC Fixation Buffer (eBioscience 00-8222-49) and permeabilized with Permeabilization Buffer (Invitrogen, 00-8333-56). Flow cytometry was performed using Accuri C6 or Sony ID7000 system and analyzed with FlowJo software.
Apc conjugated anti tnf α
Manufactured by BioLegend
Sourced in United States
APC-conjugated anti-TNF-α is a laboratory reagent used for the detection and quantification of tumor necrosis factor-alpha (TNF-α) in biological samples. It consists of an antibody specific to TNF-α that is conjugated to the fluorescent dye allophycocyanin (APC).
Automatically generated - may contain errors
Lab products found in correlation
Permeabilization buffer, by Thermo Fisher Scientific (1 mentions)
Ic fixation buffer, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd69, by BioLegend (1 mentions)
Apc conjugated anti pd 1, by BioLegend (1 mentions)
Fitc conjugated anti cd45, by BioLegend (1 mentions)
Apc conjugated anti il 2, by BioLegend (1 mentions)
Apc conjugated anti cd25, by BioLegend (1 mentions)
Apc cy7 conjugated anti cd62l, by BioLegend (1 mentions)
Pacific blue conjugated anti cd8, by BioLegend (1 mentions)
Fitc conjugated anti ifn γ, by BioLegend (1 mentions)
Id7000, by Sony (1 mentions)
Pe conjugated anti cd80, by BD (1 mentions)
Pe conjugated anti cd206, by BioLegend (1 mentions)
Apc conjugated anti tim 3, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd86, by BD (1 mentions)
Apc conjugated anti inos, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti cd80, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti il 10, by BD (1 mentions)
Pe conjugated anti tnf α, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti il 10, by BD (1 mentions)
2 protocols using apc conjugated anti tnf α
1
Comprehensive Immune Cell Profiling
2
Flow Cytometry Analysis of Decidual Immune Cells
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The following mouse-specifc mAbs were used: Pe-cy7-conjugated anti-F4/80, PE-conjugated anti-CD206, APC-conjugated anti-TNF-α (all from Biolegend, USA), APC-conjugated anti-Tim-3, FITC-conjugated anti-CD80, APC-conjugated anti-iNOS (all from eBioscience, USA), PE-conjugated anti-CD86, APC-conjugated anti-IL-10 (all from BD, USA), and APC-conjugated anti-Arg-I (RD, USA).
The following human-specific (mAbs) were used: Pe-cy7-conjugated anti-CD14, APC-conjugated anti-Tim-3, and PE-conjugated anti-TNF-α were purchased from eBioscience; FITC-conjugated anti-CD206, FITC-conjugated anti-CD163, FITC-conjugated anti-CD209, PE-conjugated anti-CD80, PE-conjugated anti-CD86, and PE-conjugated anti-IL-10 were purchased from BD company.
The mice decidual lymphocytes or human decidual macrophages were incubated with corresponding mAbs at 4°C in the dark for 30 min and were then washed once; intracellular cytokine and enzyme staining were performed after cellular fixation and permeabilization as previously described (13 (link)). Analysis was performed with a FACScantoTM II instrument (Becton Dickinson, USA).
The following human-specific (mAbs) were used: Pe-cy7-conjugated anti-CD14, APC-conjugated anti-Tim-3, and PE-conjugated anti-TNF-α were purchased from eBioscience; FITC-conjugated anti-CD206, FITC-conjugated anti-CD163, FITC-conjugated anti-CD209, PE-conjugated anti-CD80, PE-conjugated anti-CD86, and PE-conjugated anti-IL-10 were purchased from BD company.
The mice decidual lymphocytes or human decidual macrophages were incubated with corresponding mAbs at 4°C in the dark for 30 min and were then washed once; intracellular cytokine and enzyme staining were performed after cellular fixation and permeabilization as previously described (13 (link)). Analysis was performed with a FACScantoTM II instrument (Becton Dickinson, USA).
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