Log In Sign up
The largest database of trusted experimental protocols

Tunel apoptosis assay kit

Manufactured by Vazyme
Visit Supplier
Sourced in China

The TUNEL apoptosis assay kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death, in biological samples. The kit provides the necessary reagents and protocols to perform the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay, a widely used method for identifying and analyzing apoptotic cells.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)

Close spelling variants of this product

TUNEL apoptosis kit TUNEL assay TUNEL kit

3 protocols using tunel apoptosis assay kit

1

TUNEL Assay for Cell Death

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell death was detected by TUNEL staining with a TUNEL Apoptosis Assay Kit (Vazyme, Nanjing, China). The cell nuclei were stained with DAPI (5 μg/mL, Beyotime Technology, Nanjing, China) after TUNEL staining.
Shi Y., Zou Y., Xiong Y., Zhang S., Song M., An X., Liu C., Zhang W, & Chen S. (2021). Host Gasdermin D restrains systemic endotoxemia by capturing Proteobacteria in the colon of high-fat diet-feeding mice. Gut Microbes, 13(1), 1946369.
+ Open protocol
+ Expand
2

Gastric Epithelial Cell Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis of rats’ gastric epithelial cells was evaluated using TUNEL apoptosis assay kit (Vazyme, Nanjing, Jiangsu, China) according to the manufacturers’ instructions. The apoptotic cells showed intense dark nuclear staining. The proportion of the dark nuclear staining cells was evaluated by counting the cells stained with TUNEL divided by the total number of cells. One thousand nuclei were evaluated. Three independent experiments were performed blindly by two experienced pathologists.
Wu H., Wei M., Li N., Lu Q., Shrestha S.M., Tan J., Zhang Z., Wu G, & Shi R. (2020). Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157. Drug Design, Development and Therapy, 14, 5599-5610.
+ Open protocol
+ Expand
3

Quantitative TUNEL Assay of Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
According to the TUNEL apoptosis assay kit instructions (Vazyme Biotechnology, Nanjing, Suzhou, China), apoptotic cells in intestinal tissue and cultured colonocyte were detected. The nucleus was labeled with DAPI. The apoptotic cells were observed under a fluorescence microscope with 200 × or 400 × magnification and TUNEL-positive cells were counted. The images of TUNEL positive for quantification were analyzed using Image J software (NIH, Baltimore, MD, USA). Data were presented as a mean percentage of the number of TUNEL-positive cells in the whole fields of view from six randomly-selected fields in each per experiment.
Peng S., Shen L., Yu X., Wu J., Zha L., Xia Y, & Luo H. (2023). miR-200a attenuated oxidative stress, inflammation, and apoptosis in dextran sulfate sodium-induced colitis through activation of Nrf2. Frontiers in Immunology, 14, 1196065.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!