Log In Sign up
The largest database of trusted experimental protocols

Osteoassay bone plates

Manufactured by Lonza
Visit Supplier

The OsteoAssay bone plates are a specialized laboratory equipment used for the assessment of bone cell culture and bone tissue engineering applications. The plates provide a controlled environment for the cultivation and analysis of bone-related cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Tracp alp assay kit, by Takara Bio (2 mentions) Ctgf or ccn3, by Thermo Fisher Scientific (2 mentions) Microvue bone helical peptide eia assay, by Quidel (2 mentions) Image pro plus 6, by Media Cybernetics (2 mentions) Recombinant human m csf, by R&D Systems (1 mentions)

4 protocols using osteoassay bone plates

1

Quantification of Osteoclast Differentiation and Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human osteoclasts were obtained from Lonza and differentiated in vitro according to vendor’s instructions. Conditioned media collected from the KO cells or cells transfected with indicated LNAs/plasmids after 72 hours were added to osteoclast cells at day 3 of differentiation in a 6-well plate. In rescue experiments, recombinant CTGF or CCN3 (PeproTech) was supplemented in conditioned media as indicated. Cells were TRAP stained on day 7 using Acid Phosphatase, Leukocyte (TRAP) kit (Sigma-Aldrich), and TRAP+-multinucleated cells were quantified as mature osteoclasts. Quantitative TRAP assay was performed using TRACP & ALP assay kit (Takara). For osteoclast resorption function analyses, bone marrow osteoclast differentiation was conducted in OsteoAssay bone plates (Lonza) and osteoclast activity was determined by quantifying the type I collagen helical peptide α1 (I) 620–633 released from bone into culture medium using MicroVue Bone Helical Peptide EIA assay (Quidel).
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
+ Open protocol
+ Expand
2

Osteoclast Resorptive Function Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For osteoclast resorptive function analysis, the osteoclast differentiation of bone marrow macrophages/RAW264.7 cells was conducted in OsteoAssay bone plates (Lonza) at a density of 1 × 104 cells/well35 (link). Either agomir-214-3p (200 μM) or antagomir-214-3p (200 μM) were transfected on day 0 and day 5. Bone slices were ultra-sonicated in 1 mol/L NH4OH to remove adherent cells and stained with 0.1% toluidine blue solution. Bone slice images were captured using electron microscopy. Three fields were randomly selected for each bone slice for further analysis. Pit areas were quantified using Image Pro Plus 6.2 software (Media Cybernetics Inc.).
Liu J., Li D., Dang L., Liang C., Guo B., Lu C., He X., Cheung H.Y., He B., Liu B., Li F., Lu J., Wang L., Shaikh A.B., Jiang F., Lu C., Peng S., Zhang Z., Zhang B.T., Pan X., Xiao L., Lu A, & Zhang G. (2017). Osteoclastic miR-214 targets TRAF3 to contribute to osteolytic bone metastasis of breast cancer. Scientific Reports, 7, 40487.
+ Open protocol
+ Expand
3

Quantification of Osteoclast Differentiation and Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human osteoclasts were obtained from Lonza and differentiated in vitro according to vendor’s instructions. Conditioned media collected from the KO cells or cells transfected with indicated LNAs/plasmids after 72 hours were added to osteoclast cells at day 3 of differentiation in a 6-well plate. In rescue experiments, recombinant CTGF or CCN3 (PeproTech) was supplemented in conditioned media as indicated. Cells were TRAP stained on day 7 using Acid Phosphatase, Leukocyte (TRAP) kit (Sigma-Aldrich), and TRAP+-multinucleated cells were quantified as mature osteoclasts. Quantitative TRAP assay was performed using TRACP & ALP assay kit (Takara). For osteoclast resorption function analyses, bone marrow osteoclast differentiation was conducted in OsteoAssay bone plates (Lonza) and osteoclast activity was determined by quantifying the type I collagen helical peptide α1 (I) 620–633 released from bone into culture medium using MicroVue Bone Helical Peptide EIA assay (Quidel).
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
+ Open protocol
+ Expand
4

In vitro Osteoclast Differentiation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human monocytes were seeded on OsteoAssay bone plates (Lonza) at a density of 1 × 104 cells per well. The cells were cultured for three weeks in conditioned mediums from THLE-2 cells in the presence of 25 ng mL−1 recombinant human M-CSF (R&D Systems). The medium was changed every other day. On day 21, bone slices were ultra-sonicated in 1 M NH4OH to remove adherent cells and stained with 0.1% toluidine blue solution. Bone slice images were captured using electron microscopy. Three fields were randomly selected for each bone slice for further analysis. Pit areas were quantified using Image Pro Plus 6.2 software (Media Cybernetics Inc.)57 (link).
Liang C., Li J., Lu C., Xie D., Liu J., Zhong C., Wu X., Dai R., Zhang H., Guan D., Guo B., He B., Li F., He X., Zhang W., Zhang B.T., Zhang G, & Lu A. (2019). HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis. Nature Communications, 10, 4579.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!