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Pe conjugated anti iga

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PE-conjugated anti-IgA is a fluorescently labeled antibody that binds to the IgA immunoglobulin. It can be used to detect and quantify IgA in various biological samples.

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Lab products found in correlation

Fitc conjugated anti igm, by Thermo Fisher Scientific (3 mentions) Facscanto 2, by BD (2 mentions) Facscalibur, by BD (2 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Cellquest, by BD (1 mentions)

Close spelling variants of this product

PE‐conjugated anti‐human IgA PE-conjugated anti-mouse IgA Anti‐IgA‐PE PE-IgA

4 protocols using pe conjugated anti iga

1

IgA Class Switching in CH12 Cells

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CH12 cells were cultured and stimulated with 250ng/mL CD40L, 10ng/mL IL4 and 1ng/mL TGFβ (CIT) to induce class switching to IgA. For siRNA-based knockdown experiments, transfection of siRNA oligonucleotide (Invitrogen) into CH12 cells was done using electroporation kit (Lonza). The cells were subsequently cultured for 24 hours before the addition of CIT and incubated further for an additional 24 hours. For FACS analysis, the cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech). All analyses were performed on FACS Calibur (Becton Dickinson).
Gupta R., Somyajit K., Narita T., Maskey E., Stanlie A., Kremer M., Typas D., Lammers M., Mailand N., Nussenzweig A., Lukas J, & Choudhary C. (2018). DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell, 173(4), 972-988.e23.
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2

Mouse B Lymphocyte Class Switching

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The mouse B lymphocyte cell line CH12-F3 cells was cultured and stimulated with 250 ng/ml CD40L, 10 ng/ml IL4 and 1 ng/ml TGFβ (CIT) to induce immunoglobulin class switching from IgM to IgA. For FACS analysis of CSR efficiency, live CH12-F3 cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech) antibodies. All analyses were performed on FACS CantoII (BD Biosciences).
Shinoda K., Zong D., Callen E., Wu W., Dumitrache L.C., Belinky F., Chari R., Wong N., Ishikawa M., Stanlie A., Multhaupt-Buell T., Sharma N., Ozelius L., Ehrlich M., McKinnon P.J, & Nussenzweig A. (2021). The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice. Molecular cell, 81(12), 2611-2624.e10.
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3

Mouse B-cell Lymphoma IgM to IgA Switching

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The mouse B-cell lymphoma line CH12F3-2A expressing Bcl2 (39 (link)) was cultured in complete RPMI supplemented with 5% NCTC, 10% FBS and 0.05% 2-mercaptoethanol as described. For IgM to IgA isotype switch analysis, CH12F3-2A cells were stimulated with CIT (CD40-L, IL-4 and TGF-β) and harvested after 24 or 48 hours of induction as previously described (40 (link)). Cells were surface stained with FITC-conjugated anti-IgM (eBioscience) and PE-conjugated anti-IgA (Southern Biotechnology) antibodies. Flow cytometric analysis was performed using FACS-Calibur and CellQuest software (BD Biosciences). The antibodies used for staining are shown in Supplementary Table S1.
Gothwal S.K., Refaat A.M., Nakata M., Stanlie A., Honjo T, & Begum N.A. (2024). BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL. Nucleic Acids Research, 52(8), 4422-4439.
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4

Induced IgM to IgA Class Switching

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The mouse B lymphocyte cell line CH12-F3 cells was cultured and stimulated with 250 ng/ml CD40L, 10 ng/ml IL4 and 1 ng/ml TGFb (CIT) to induce immunoglobulin class switching from IgM to IgA. For FACS analysis of CSR efficiency, live CH12 cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech) antibodies. All analyses were performed on FACS CantoII (BD Biosciences).
Shinoda K., Zong D., Callén E., Wu W., Dumitrache L.C., Belinky F., Wong N., Ishikawa M., Stanlie A., Ehrlich M.E., McKinnon P.J., & Nussenzweig A. (2020). The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice.
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