Cells were lysed using Triton X-100 sample buffer (Sigma-Aldrich), and proteins were separated by SDS-PAGE. Detection occurred by using specific antibodies against GAPDH (1:1000 dilution, #2275-PC-100, Trevigen), SARS-CoV-2 NP (1:1000 dilution, #40143-R019, Sino Biological), ACE2 (1:500 dilution, #ab15348, Abcam), and TMPRSS2 (1:1000 dilution, Recombinant Anti-TMPRSS2 antibody [EPR3861], #ab92323, Abcam) followed by incubation with IRDye-labeled secondary antibodies (LI-COR Biotechnology, IRDye800CW Goat anti-Rabbit, 926-32211, 1:40,000) according to the manufacturer’s instructions. Protein bands were visualized by laser-induced fluorescence using an infrared scanner for protein quantification (Odyssey, Li-Cor Biosciences, Bad Homburg, Germany).
Triton x 100 sample buffer
Manufactured by Merck Group
Triton X-100 sample buffer is a non-ionic detergent commonly used in biological and biochemical applications. It is designed to solubilize and denature proteins while maintaining their native structure and activity. The buffer helps to extract and prepare samples for various analytical techniques.
Automatically generated - may contain errors
2 protocols using triton x 100 sample buffer
1
SARS-CoV-2 Protein Detection by Western Blot
2
SARS-CoV-2 Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed using Triton-X-100 sample buffer (Sigma-Aldrich), and proteins were separated by SDS-PAGE. Detection occurred by using specific antibodies against SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit monoclonal antibody (Mab), #40143-R019, Sino Biological), ACE2 (1:500 dilution, Anti-ACE2 antibody, #ab15348, Abcam, Berlin, Germany), TMPRSS2 (1:1000 dilution, Recombinant Anti-TMPRSS2 antibody [EPR3861], #ab92323, Abcam), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen, Wiesbaden, Germany). Protein bands were visualized by laser-induced fluorescence using an infrared scanner for protein quantification (Odyssey, Li-Cor Biosciences, Bad Homburg, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!