Invitrogen igg total human uncoated elisa kit

For murine sera, plates were coated with WPE (20 μg/mL) or Ara h 1, Ara h 2, or Ara h 3 (5 μg/mL) diluted in carbonate-bicarbonate buffer at pH 9.6 (Sigma-Aldrich, St Louis, Mo). Samples were assayed at 1:10 or 1:20 (IgE) or 1:500 (IgG₁). Assays were performed as previously described.²⁷ Units reported as titers were conducted as previously described.^{26 (link)} Human Ara h 1–specific IgG was quantified using the Invitrogen IgG (Total) Human Uncoated ELISA Kit (Thermo Fisher Scientific). Plates were coated with Ara h 1 (5 μg/mL) to assay allergen-specific polyclonal IgG. Absorbances were measured and concentrations were calculated using the Biotek Gen5 Microplate Reader and Imager Software (Biotek, Winooski, Vt).