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Angiotensin 2 converting enzyme ace2 activity assay kit fluorometric

Manufactured by Abcam
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The Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) is a laboratory tool used to measure the activity of the ACE2 enzyme. The kit utilizes a fluorometric method to detect and quantify the enzymatic activity of ACE2.

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Synergy h1, by Agilent Technologies (1 mentions)

Close spelling variants of this product

ACE2 Activity Assay Kit (Fluorometric) ACE2 activity assay kit ACE2 activity kit ACE2 assay kit Enzyme activity kit Fluorometric assay kit Angiotensin II Activity assay kits Enzyme assay kits Fluorometric assays

2 protocols using angiotensin 2 converting enzyme ace2 activity assay kit fluorometric

1

Assessing ACE2 Enzymatic Activity

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ACE2 enzymatic activity was assessed using the Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (Abcam, catalog number ab273297). Recombinant ACE2 enzymatic activity was assessed at 37°C over 3 h (Ex320 nm/Em420 nm kinetic read; BioTek Synergy H1) in the presence or absence of treatment with 25 μM bifonazole. Similarly, the effect of bifonazole on inhibition of ACE2 enzymatic activity by a defined ACE2 inhibitor was assessed. To confirm the enzymatic activity of ACE2 in the model cell line VeroAT, cells were harvested according to the manufacturer’s protocol, and cellular extracts were assayed at 37°C over 3 h.
Taha Z., Arulanandam R., Maznyi G., Godbout E., Carter-Timofte M.E., Kurmasheva N., Reinert L.S., Chen A., Crupi M.J., Boulton S., Laroche G., Phan A., Rezaei R., Alluqmani N., Jirovec A., Acal A., Brown E.E., Singaravelu R., Petryk J., Idorn M., Potts K.G., Todesco H., John C., Mahoney D.J., Ilkow C.S., Giguère P., Alain T., Côté M., Paludan S.R., Olagnier D., Bell J.C., Azad T, & Diallo J.S. (2022). Identification of FDA-approved bifonazole as a SARS-CoV-2 blocking agent following a bioreporter drug screen. Molecular Therapy, 30(9), 2998-3016.
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2

Vero ACE2 Protein Characterization and Activity Assay

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Vero ACE2 protein sequence was obtained from NCBI annotation of our assembly (NCBI Chlorocebus sabaeus (Vero cell) Annotation Release 102 (AR 102)) and a BLAST18 (link) search was run on the NCBI portal to identify residues mutations. The 3D structure of vACE2 was modeled using Phyre2 server39 (link), mutations were marked using EzMol40 (link).
For ACE2 activity assessment, three separate cultures of Vero cells were prepared in the same conditions and used with Abcam Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297) as per the provided protocol all conditions were analysed in triplicates. Fluorometric reading was performed for an hour at 1 min intervals.
Sène M.A., Kiesslich S., Djambazian H., Ragoussis J., Xia Y, & Kamen A.A. (2021). Haplotype-resolved de novo assembly of the Vero cell line genome. NPJ Vaccines, 6, 106.
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