Acquity 1 classtm

Human recombinant CYP2J2 was purchased from Cypex (Scotland, UK). β-nicotinamide adenine dinucleotide phosphate disodium salt (NADP⁺), D-glucose-6-phosphate, (G-6-P) glucose-6-phosphatedehydrogenase were purchased from Sigma (Darmstadt, Germany). All herbal medicines were obtained from Beijing Tong Ren Tang (China, Beijing). All commercially available reagents and solvents were used as received. ¹H NMR spectra were recorded at 400 MHz and ¹³C NMR data were collected at 100 MHz with complete proton decoupling. ESI-HRMS spectra of all compounds were recorded by Synapt G2-Si^TM (Q-TOF-MS) equipped with a high-pressure liquid chromatography (Waters Acquity I-Class^TM). Flash column chromatography was carried out on silica gel (300–400 mesh, Qingdao Marine Chemical Ltd, Qingdao, China). Thin-layer chromatography (TLC) was performed on TLC silica gel 60 F254 plates. The purities of all final compounds were determined by HPLC to be above 95%.

All commercially available reagents and solvents were used as received. 1H-NMR spectra were recorded at 400 MHz and 13 C-NMR data were collected at 100 MHz with complete proton decoupling. ESI-HRMS spectra of all compounds were recorded by Synapt G2-SiTM (Q-TOF-MS) equipped with a high-pressure liquid chromatography (Waters Acquity I-ClassTM). Flash column chromatography was carried out on silica gel (300–400 mesh, Qingdao Marine Chemical Ltd, Qingdao, China). Thin layer chromatography (TLC) was performed on TLC silica gel 60 F254 plates. Melting points were uncorrected and determined on Shenguang melting point apparatus (SGW X-4). The purities of all final compounds were determined by HPLC to be above 95%. HPLC instrument: Waters 2695/2996 HPLC (Column: YMC-Pack ODS-A C18 (4.6 × 150 mm, 5 μm), S/N：109DA80059. Elution: MeOH in water; Flow rate: 1.0 ml/min.