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Neon transfection system device

Manufactured by Thermo Fisher Scientific
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The Neon Transfection System is a laboratory device designed to facilitate the introduction of nucleic acids, such as DNA or RNA, into mammalian cells. It employs an electroporation-based technique to temporarily increase the permeability of cell membranes, allowing the efficient uptake of the desired genetic material.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (6 mentions) Neon transfection system 100 μl kit, by Thermo Fisher Scientific (3 mentions) Mpk10025, by Thermo Fisher Scientific (1 mentions) Pgl3 promoter vector, by Promega (1 mentions) Dual luciferase reporter assay kit, by Promega (1 mentions)

Close spelling variants of this product

Neon transfection device Neon Transfection System Neon transfection Neon device Neon system NeonTM

4 protocols using neon transfection system device

1

Enhancer-Driven Luciferase Assay in Jurkat Cells

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We performed reporter assays using a pGL3-Promoter Vector (E1761, Promega) luciferase construct alone and coupled with human and mouse PE enhancer sequences (hPE: hg19; 90,144,758–90,149,109; mPE: mm10; chr19:33,220,867–33,224,929; both sequences were synthesized by Genewiz, RRID:SCR_003177), cloned in the forward and reverse orientations. In these assays, we electroporated JURKAT cells with a Neon Transfection System Device (MPK5000, Thermo Fisher Scientific) using 100μL tips (MPK10025, Thermo Fisher Scientific). Electroporation conditions: pulse voltage 1350v, pulse width 10ms, pulse number 3, cell density 107/ml. PE constructs were transfected together with a plasmid driving the expression of the Renilla luciferase gene (pCMV-Renilla) used as an internal control. We measured luciferase activity 42h after electroporation with the Dual-Luciferase Reporter Assay kit (E1980, Promega).
Tottone L., Lancho O., Loh J.W., Singh A., Kimura S., Roels J., Kuchmiy A., Strubbe S., Lawlor M.A., da Silva-Diz V., Luo S., Gachet S., García-Prieto C.A., Hagelaar R., Esteller M., Meijerink J.P., Soulier J., Taghon T., Van Vlierberghe P., Mullighan C.G., Khiabanian H., Rocha P.P, & Herranz D. (2020). A Tumor Suppressor Enhancer of PTEN in T-cell Development and Leukemia. Blood Cancer Discovery, 2(1), 92-109.
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2

Electroporation and Lipofectamine Transfection

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HH-B2 cells were transfected by electroporation using the Neon Transfection System device (no. MPK5000; Thermo Fisher Scientific). Briefly, 4 × 106 cells were mixed with 10 μg of plasmid DNA in 100 μL of buffer B supplied in the Neon Transfection System 100 μL kit (no. MPK10096; Thermo Fisher Scientific) and then electroporated using the setting of 1,400 V/20 ms/2 pulses. DNA transfection in 293T or HKB5/B5 cells was carried out using Lipofectamine 2000 according to the manufacturer’s instruction (no. 11668019; Thermo Fisher Scientific).
Chen L.Y., Chen L.W., Hung C.H., Lin C.L., Wang S.S, & Chang P.J. (2022). Caspase-Mediated Cleavage of the Transcription Factor Sp3: Possible Relevance to Cancer and the Lytic Cycle of Kaposi’s Sarcoma-Associated Herpesvirus. Microbiology Spectrum, 10(1), e01464-21.
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3

Electroporation-based Plasmid Transfection

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All lymphoma cell lines were transfected by electroporation using the Neon Transfection System device (catalog no. MPK5000; Thermo Fisher Scientific, Waltham, MA, USA) and the Neon Transfection System 100 μL kit (catalog no. MPK10096; Thermo Fisher Scientific, Waltham, MA, USA). Briefly, 4 × 106 cells, along with 10 or 20 μg of plasmid DNA, were resuspended in 100 μL of Buffer B (supplied in the Neon Transfection System 100 μL kit). After electroporation (1400 V, 20 ms, 2 pulses), the transfected cells were transferred into the culture medium (2 mL) in 6-well plates. Transient DNA transfection in 293T cells was performed using Lipofectamine 2000 according to the manufacturer’s instruction (catalog no. 11668019; Thermo Fisher Scientific, Waltham, MA, USA).
Chen L.W., Wang S.S., Hung C.H., Hung Y.H., Lin C.L, & Chang P.J. (2021). The Epstein-Barr Virus Lytic Protein BMLF1 Induces Upregulation of GRP78 Expression through ATF6 Activation. International Journal of Molecular Sciences, 22(8), 4024.
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4

Electroporation and Lipofectamine Transfection

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HH-B2 cells were transfected by electroporation using the Neon Transfection System device (no. MPK5000; Thermo Fisher Scientific). Briefly, 4 × 106 cells were mixed with 10 μg of plasmid DNA in 100 μL of buffer B supplied in the Neon Transfection System 100 μL kit (no. MPK10096; Thermo Fisher Scientific) and then electroporated using the setting of 1,400 V/20 ms/2 pulses. DNA transfection in 293T or HKB5/B5 cells was carried out using Lipofectamine 2000 according to the manufacturer’s instruction (no. 11668019; Thermo Fisher Scientific).
Chen L.Y., Chen L.W., Hung C.H., Lin C.L., Wang S.S, & Chang P.J. (2022). Caspase-Mediated Cleavage of the Transcription Factor Sp3: Possible Relevance to Cancer and the Lytic Cycle of Kaposi’s Sarcoma-Associated Herpesvirus. Microbiology Spectrum, 10(1), e01464-21.
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