Anti tfe3

Manufactured by Cell Signaling Technology

Anti-TFE3 is an antibody product developed by Cell Signaling Technology. It is designed to detect the transcription factor TFE3 protein in various biological samples using techniques such as Western blotting and immunohistochemistry.

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3 protocols using anti tfe3

Western Blot Protein Analysis Protocol

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The procedures for protein sample preparation from cell cultures, protein quantification, Western blot, and data analysis were performed as previously described.16 (link) The following antibodies were used for Western blot analyses: anti-TFE3 (Cell Signaling Technology (CST), catlog number 14779), anti-β-actin (Sigma, A3854), anti-HK2 (CST, catlog number 2867), anti-CHOP (CST, catlog number 2895). Protein bands were visualized by chemiluminescence using an Enhanced chemiluminescence detection kit (Thermo Scientific, 32106). The band density was quantified by densitometry using Image J software, and individual protein band density was normalized with respect to β-actin levels.

Wang Z., Xu F., Hu J., Zhang H., Cui L., Lu W., He W., Wang X., Li M., Zhang H., Xiong W., Xie C., Liu Y., Zhou P., Liu J., Huang P., Qin X.F, & Xia X. (2021). Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity. Journal for Immunotherapy of Cancer, 9(5), e002155.

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Comprehensive Antibody Catalog for Research

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The following antibodies were used: anti-Tsg101 (Cat. no. ab83) and anti-Vps28 (Cat. no. ab167172) from Abcam; anti-TFEB (Cat. no. 4240S), anti-P-TFEB (S122, Cat. no. 86843 and S211, Cat. no. 37681), anti-TFE3 (Cat. no. 14779S), anti-P-Ulk1 (S757, Cat. no. 6888), anti-P-4E-BP1 (Thr37/46, Cat. no. 2855), anti-P-S6K1 (Thr389, Cat. no. 9234), anti-mTOR (Cat. no. 2983), and anti-HA-Tag (Cat. no. 2367) from Cell Signaling Technologies; anti-LAMP1 (Cat. no. H4A3) from DSHB; anti-mono- and -polyubiquitinylated conjugates (Cat. no. BML-PW8810) from Enzo Life Sciences; anti-GFP (Cat. no. AF4240) from R&D Systems; anti-GAPDH (Cat. no. sc-25778) and anti-lamin A/C (Cat. no. sc-7292) from Santa Cruz; anti-histone H3 (Cat. no. H0164), anti-LAMP1 (Cat. no. L-1418), anti-vinculin (Cat. no. V9131-.2ML), and anti-tubulin (Cat. no. T5168) from Sigma-Aldrich; secondary HRP-conjugated goat anti-mouse, goat anti-rabbit, and bovine anti-goat antibodies from Jackson ImmunoResearch; and secondary Alexa Fluor 488–conjugated donkey anti-mouse and Alexa Fluor 647–conjugated donkey anti-rabbit antibodies from Thermo Fisher Scientific.

Wróbel M., Cendrowski J., Szymańska E., Grębowicz-Maciukiewicz M., Budick-Harmelin N., Macias M., Szybińska A., Mazur M., Kolmus K., Goryca K., Dąbrowska M., Paziewska A., Mikula M, & Miączyńska M. (2022). ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathway. Life Science Alliance, 5(7), e202101239.

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Immunoblotting for Intracellular Trafficking Proteins

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The following antibodies were used: anti-Tsg101 (Cat# ab83), anti-Vps28 (Cat# ab167172) from Abcam; anti-TFEB (Cat # 4240S), anti-TFE3 (Cat# 14779S) from Cell Signaling Technologies; anti-LAMP1 (Cat# H4A3) from DSHB; anti-mono-and -polyubiquitinylated conjugates (Cat# BML-PW8810) from Enzo Life Sciences; anti-GFP (Cat# AF4240) from R&D Systems; anti-GAPDH (Cat# sc-25778) from Santa Cruz; anti-histone H3 (Cat# H0164), anti-LAMP1 (Cat# L-1418), anti-vinculin (Cat# V9131-.2ML) from Sigma; secondary horseradish peroxidase (HRP)conjugated goat anti-mouse, goat anti-rabbit, and bovine anti-goat antibodies (Jackson ImmunoResearch); secondary Alexa Fluor 488-conjugated donkey anti-mouse and Alexa Fluor 647-conjugated donkey anti-rabbit antibodies from Thermo Fisher Scientific.

Szymańska E., Szymańska E., Budick-Harmelin N., Kolmus K., Goryca K., Dąbrowska M., Paziewska A., Mikula M., Cendrowski J., & Miączyńska M. (2021). ESCRT-I controls lysosomal membrane protein homeostasis and restricts MCOLN1-dependent TFEB/TFE3 signaling.

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