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2 protocols using 15 acetyldeoxynivalenol

1

Minimal Medium for DON Degradation

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Minimal medium (MM) was used as a culture medium for the enrichment and isolation of DON-degrading strains. The composition of MM (pH 7.0) was described by Wang et al. (2019b) (link). Ingredients were purchased from Sigma-Aldrich (Oakville, Ontario, Canada), Guanghua Sci-Tech Co., Led. (Shantou, Guangdong, China), XILONG SCIENTIFIC (Shantou, Guangdong, China), and Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Toxin standards for DON, HT-2, T-2, 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON) were purchased from Romer Labs (Erber Campus, Getzersdorf, Austria).
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2

Comprehensive Analysis of Mycotoxins in Chinese Grains

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AFB1, AFB2, AFG1, AFG2, AFM1, AFM2, ochratoxin A (OTA), ochratoxin α (OTα), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), neosolaniol (NEO), fusarenon X (Fus X), citrinin (CIT), fumonisins B1 (FB1), fumonisins B2 (FB2), zearalenone (ZEN), zearalanone(ZAN), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL) were purchased from Romer Labs, Inc. (Union, MO, USA). Sterigmatocystin (STM), gliotoxin (GLI), cyclopiazonic acid (CPA), patulin (PAT), alternariol (AOH), alternariolmethyether (AME) and altenuisin (ALS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, hexane, acetone, formic acid, chloroauric acid, cysteine and ammonium acetate were also obtained from Sigma-Aldrich. Milli-Q water (Millipore, Billerica, MA, USA) was used for all analyses.
A total of 40 samples, including 20 peanut samples, 10 wheat samples, and 10 maize samples, were purchased from bazaars in Shanghai, China.
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