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Rna quick and easy kit

Manufactured by Qiagen
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The RNA Quick and Easy Kit is a laboratory equipment product designed for the rapid and efficient extraction and purification of RNA from various sample types. It provides a straightforward and streamlined process for isolating high-quality RNA, which is a fundamental step in various molecular biology applications.

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Lab products found in correlation

Facsaria 3 sorter, by BD (2 mentions) Nylon mesh cell strainer snap caps, by STEMCELL (2 mentions) Tryple, by Thermo Fisher Scientific (2 mentions) Facsdiva version 8, by BD (2 mentions)

2 protocols using rna quick and easy kit

1

Fluorescence-Activated Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were incubated for 72 h post-transfection. Before sorting, cells were washed with phosphate-buffered saline (PBS) and incubated with TrypLE (Gibco) until they detached. After two washing steps with PBS, cells were resuspended in FACS Buffer (PBS with 2% FBS and 2 mM EDTA) and filtered through 35 μm nylon mesh cell strainer snap caps (Stemcell technologies). Flow cytometry was done on a FACSAria III sorter (BD Biosciences) using FACSDiva version 8.0.1 (BD Biosciences). Gating is shown in Suppl. Fig. 29, 30. RNA from sorted cells was extracted using Qiagen RNA Quick and easy kit according to the manufacturer’s instructions.
Villiger L., Rothgangl T., Witzigmann D., Oka R., Lin P.J., Qi W., Janjuha S., Berk C., Ringnalda F., Beattie M.B., Stoffel M., Thöny B., Hall J., Rehrauer H., van Boxtel R., Tam Y.K, & Schwank G. (2021). In vivo cytidine base editing of hepatocytes without detectable off-target mutations in RNA and DNA. Nature biomedical engineering, 5(2), 179-189.
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2

Fluorescence-Activated Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were incubated for 72 h post-transfection. Before sorting, cells were washed with phosphate-buffered saline (PBS) and incubated with TrypLE (Gibco) until they detached. After two washing steps with PBS, cells were resuspended in FACS Buffer (PBS with 2% FBS and 2 mM EDTA) and filtered through 35 μm nylon mesh cell strainer snap caps (Stemcell technologies). Flow cytometry was done on a FACSAria III sorter (BD Biosciences) using FACSDiva version 8.0.1 (BD Biosciences). Gating is shown in Suppl. Fig. 29, 30. RNA from sorted cells was extracted using Qiagen RNA Quick and easy kit according to the manufacturer’s instructions.
Villiger L., Rothgangl T., Witzigmann D., Oka R., Lin P.J., Qi W., Janjuha S., Berk C., Ringnalda F., Beattie M.B., Stoffel M., Thöny B., Hall J., Rehrauer H., van Boxtel R., Tam Y.K, & Schwank G. (2021). In vivo cytidine base editing of hepatocytes without detectable off-target mutations in RNA and DNA. Nature biomedical engineering, 5(2), 179-189.
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