Levels of LDH were measured by an automated clinical analyzer in the University Hospital of Ghent. Cytokine levels of IL-6 and chemokine (C-X-C motif) ligand 1 (CXCL-1) were determined with Luminex magnetic bead-based assays (R&D Systems, Biotechne). Plasma P-selectin levels were measured through a Quantikine ELISA kit (R&D Systems, Biotechne).
Luminex magnetic bead based assays
Manufactured by R&D Systems
Sourced in United Kingdom
Luminex magnetic bead-based assays are a multiplex technology that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The assays utilize polystyrene beads that are dyed with fluorescent colors and coated with capture antibodies specific to the target analytes. These beads are then combined with the sample, and the target analytes bind to their respective capture antibodies. The beads are then analyzed using a Luminex instrument, which uses the fluorescent signatures to identify and quantify the captured analytes.
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Lab products found in correlation
2 protocols using luminex magnetic bead based assays
1
Biomarker Profiling in Clinical Settings
2
Cytokine and Ezrin Profiling in Vitreous
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Vitreous levels of C‐C motif chemokine ligand (CCL)2, CCL17, CD40, C‐X‐C motif chemokine ligand (CXCL)13, FASL, IL‐6 and IL‐10 were measured by Luminex magnetic bead‐based assays (R&D Systems Europe, Abingdon, United Kingdom). Ezrin (EZR) was measured by ELISA (LSBio, Seattle, WA, USA). The assays were conducted according to the manufacturer’s instructions.
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