The largest database of trusted experimental protocols

2 protocols using 8s hete

1

Recombinant Eicosanoid Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
ARA, EPA, and DHA were purchased from Nacalai Tesque (Kyoto, Japan). (±)8-HEPE (comprising equal amounts of 8S-HEPE and 8R-HEPE), 8S-HEPE, (±)8-HETE, 8S-HETE, 8R-HETE, rac-10-HDHA, PD1, gamma-linoleic acid (GLA), stearidonic acid (SDA) and docosapentaenoic acid (DPA) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). The Spodoptera frugiperda (Sf9) insect cells, and pFastBac vector were purchased from Thermo Fisher Scientific (Franklin, MA, USA). The anti-6xHis antibody [HIS.H8] was purchased from Abcam Japan (Tokyo, Japan).
+ Open protocol
+ Expand
2

Metabolite Confirmation Standards for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemical standards utilized for the confirmation of identified metabolites included glycocholic acid (sc-218574), glycoursodeoxycholate (sc-211567), and glycolithocholate (sc-396741) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA); glycochenodeoxycholate (16942), taurodeoxycholate (15935), acetylcarnitine (16948), octanoylcarnitine (15048), prostaglandin B2 (11210), 12-oxo-leukotriene B4 (20140), arachidonic acid (90010), 12(R)-HETE (34560), 8(S)-HETE (34360) and lyso-PAF (C18) (60916) from Cayman Chemical (Ann Arbor, Michigan, USA); glycodeoxycholate (G9910), taurochenodeoxycholate (T6260), lysophosphatidylcholine (L4129) and from Sigma-Aldrich (St. Louis, MO, USA); and Sphingomyelin mixture (860061,2,3, Avanti Polar Lipids, Inc., Alabaster, Alabama, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!