Anti adrp

General laboratory chemicals were obtained from Sigma-Aldrich or Merck Millipore. Anti-β-ACTIN and anti-HA antibodies were purchased from Sigma-Aldrich. Anti-H3K27me3, anti-EZH2, anti-JMJD3, anti-MUSASHI (MSI), anti-NUMB, anti-NOTCH1, anti-Cleaved Notch1 (Val1744) (NICD), anti-Tyr485 p85/ Tyr199 p55 phospho-PI3K, anti-Ser2448 phospho-mTOR, anti-Thr389 phospho-p70S6K and anti-Ser536 phospho-NF-κB p65 were purchased from Cell Signaling Technology. Anti-ADRP, anti-CD36, anti-ABCA1, anti-SMRTe and anti-MINT (SPEN) were purchased from Santa Cruz Biotechnology, Inc. HRP conjugated anti-rabbit IgG and anti-mouse IgG and anti-rabbit DyLight 488 were obtained from Jackson ImmunoResearch. Fluorescein isothiocyanate (FITC)-conjugated monoclonal antibodies (mAbs) to mouse MHC class II, phycoerythrin (PE)-conjugated mAbs to mouse F4/80 were from BD Biosciences. Anti-mouse CD19-APC and CD3-FITC were from Imgenex. Ziehl-Neelsen (ZN) staining Kit was purchased from HiMedia and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was from Sigma-Aldrich. BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) and HCS LipidTOX Red neutral lipid stain was from Molecular Probes (Invitrogen/Thermo Fisher Scientific).

Primary antibodies used: anti-ADRP (Santa Cruz, Cat# sc-377429), anti-GFP (Roche, Cat# 11814460001), anti-β-actin (Sigma, Cat# A1978), anti-β-tubulin (Sigma, Cat# T4026), and anti-Tom20 (Santa Cruz, Cat# sc-17764). Secondary antibodies used: Alexa Fluor 594 donkey anti-rabbit (Invitrogen, Cat# A21207), Alexa Fluor 594 donkey anti-mouse (Invitrogen, Cat# A21203), Alexa Fluor 633 donkey anti-mouse (Invitrogen, Cat# A21050), Alexa Fluor 647 donkey anti-rabbit (Invitrogen, Cat# A31573), Alexa Fluor 647 donkey anti-mouse (Invitrogen, Cat# A31571), Alexa Fluor 680 donkey anti-rabbit (Invitrogen, Cat# A10043), and Alexa Fluor 800 donkey anti-mouse (Invitrogen, Cat# A32789). Reagents used were: DAPI (Invitrogen, Cat# B223), Hoechst-33342 (Sigma, Cat# B2261), HCS LipidTOX Deep Red (Thermo Fisher Scientific, Cat# H34477), Mitotracker Green (Thermo Fisher Scientific, Cat# M7514), CellROX Deep Red (Thermo Fisher Scientific, Cat# C10422), Image-iT Lipid Peroxidation Kit (Thermo Fisher Scientific, Cat# C10445), CellTrace Calcein Green, AM (Thermo Fisher Scientific, Cat# C34852), FluorSave reagent (Millipore, Cat# 345789-20), Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific, Cat# 13778075), and Lipofectamine 3000 reagent (ThermoFisher Scientific, Cat# L3000015).