Agilent 6540 q tof instrument

Solvents and reagents were provided by Bide Pharmatech (Beijing, China). The commercial herbicide picloram was provided by Nutrichem Company Ltd. (Beijing, China). Arabidopsis thaliana (A. thaliana ecotype Columbia-0, Col-0) and weed seeds were provided by the Laboratory of National Forestry and Grassland Administration on Pest Chemical Control, China Agricultural University, Beijing, China. ¹H NMR and ¹³C NMR spectra were obtained at 500 MHz using a Bruker AVANCE NOE500 spectrometer (Billerica, MA, USA) in DMSO-d₆ solution. HRMS was performed using an Agilent 6540 Q-TOF instrument (Santa Clara, CA, USA) instrument. The A. thaliana and weed root growth data were obtained using IMAGEJ software (https://imagej.nih.gov/ij/).

All solvents were obtained from commercial sources and were used without further processing. The melting points of all the compounds were determined using a B-III microscope (Beijing Technical Instrument Co., Beijing, China) and were uncorrected. Nuclear magnetic resonance (NMR) spectra were recorded using a Bruker AM-500 spectrometer. High-resolution mass spectra (HRMS) were obtained using an Agilent 6540 QTOF instrument.