Hematoxylin and eosin h e staining

The main organs (heart, liver, spleen, lung, kidney and intestine) of healthly BALB/c nude mice (~8 weeks old, 18 g) were collected under euthanasia after treatment with PBS and Gi-F-CAA (8, 12 and 16 mg/kg in 100 μL PBS) for 1 day. Afterwards, the histology evaluation by Hematoxylin and Eosin (H&E) Staining was carried out by Servicebio Technology Co., Ltd. (Wuhan, China). Meanwhile, the whole blood and serum of healthly BALB/c nude mice (~8 weeks old, 18 g) were collected after treatment with PBS and Gi-F-CAA (8, 12 and 16 mg/kg in 100 μL PBS) for 1 days. The blood biochemistry analysis was performed with Hitachi Automatic Biochemical Analyzer 7100. The blood routine analysis was carried out by Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

The rats were euthanized 28 d after surgery. The hearts were quickly excised and fixed in paraformaldehyde. Infarct size and LV wall thickness in the MI zone were detected by Masson staining (Servicebio, Wuhan, China). Hematoxylin and eosin (H&E) staining (Servicebio, Wuhan, China) was used to observe the tissue morphology of the MI area and toxicity in other organs.