Log In Sign up
The largest database of trusted experimental protocols

Gpr37

Manufactured by Abcam
Visit Supplier
Sourced in United States

GPR37 is a G protein-coupled receptor that is expressed in the central nervous system. It plays a role in cellular signaling pathways, but its exact function is not fully characterized.

Automatically generated - may contain errors

Lab products found in correlation

Core data acquisition system, by Cytiva (1 mentions) A confocal laser scanning microscope, by Zeiss (1 mentions) Adam17, by Abcam (1 mentions) P egfr, by GeneTex (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Gapdh, by Abcam (1 mentions)

2 protocols using gpr37

1

Immunofluorescent Staining and Confocal Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescent staining, the cells were incubated with primary antibodies against REG4 (R&D) and GPR37 (Abcam), followed by incubation with iFluor 594 anti-rabbit IgG antibody (AAT Bioquest) or Alexa488-conjugated anti-goat IgG antibody (Life technologies). For confocal microscopy, the cells on cover-slips were counterstained with DAPI and imaged using a confocal laser-scanning microscope (Carl Zeiss) with a core data acquisition system (Applied Precision).
Wang H., Hu L., Zang M., Zhang B., Duan Y., Fan Z., Li J., Su L., Yan M., Zhu Z., Liu B, & Yang Q. (2016). REG4 promotes peritoneal metastasis of gastric cancer through GPR37. Oncotarget, 7(19), 27874-27888.
+ Open protocol
+ Expand
2

Immunoblotting Profiling of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracts were resolved through 8%~15% SDS-PAGE, transferred to PVDF membranes, and probed with antibodies against human REG4 (R&D), EGFR(CST, USA), p-EGFR(GeneTex, USA), ERK(CST, USA), p-ERK(CST, USA), SP1(CST, USA), p-SP1(GeneTex, USA), GPR37(Abcam, USA), ADAM17(Abcam, USA), p-ADAM17(Abcam, USA) and GAPDH (Abcam, USA). Peroxidase-conjugated anti-mouse or rabbit IgG (CST, USA) was used as secondary antibody, and the antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL, Thermo).
Wang H., Hu L., Zang M., Zhang B., Duan Y., Fan Z., Li J., Su L., Yan M., Zhu Z., Liu B, & Yang Q. (2016). REG4 promotes peritoneal metastasis of gastric cancer through GPR37. Oncotarget, 7(19), 27874-27888.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!