Icp aes icap 7400

The concentration of freshly prepared metallothionein-2 was measured using 1 mM DTNB with 1 mM EDTA in 50 mM borate buffer, pH 7.4, with 100 mM NaCl. The absorbance was measured in kinetic mode at 412 nm. The absorbance at the plateau was used for concentration calculation.^{58 (link)} Metal content was assessed using a solution containing 200 µM PAR with 1 mM DTNB in 50 mM borate buffer, pH 7.4, with 100 mM NaCl. The absorbance was measured in kinetic mode at 492 nm. The absorbance at the plateau was used for concentration calculation.^{59 (link)} Additionally, the samples were analyzed by ICP-AES iCAP 7400, Thermo Scientific to confirm the spectrophotometric results. Prior to analysis, protein samples were diluted with 0.5 M nitric acid.