Human breast cancer cell line MDA-MB-231 was obtained from the America Type Culture Collection (ATCC, Manassas, VA). The cell lines were prepared as previously described [47 (link)]. For the experiments, cells were trypsinized, and one million cells were counted and washed with 1X PBS; cell pellets were frozen at −80 °C. A total of three replicates were prepared for each cell types. Ethanol, pyridine, methoxyamine hydrochloride, C8−C30 fatty acid methyl esters (FAMEs), and ammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO). Liquid chromatography−mass spectrometry (LC−MS) Optima-grade methanol and acetonitrile, formic acid, N-methyl-N-trimethyl –silyl-tri-fluoro-acetamide (MSTFA) with 1% TMCS, and hexane were obtained from Fisher Sci. (Fair Lawn, NJ). The ultrapure water was produced by Millipore Advantage A10 system with an LC−MS Polisher filter (Billerica, MA).
C8 c30 fatty acid methyl esters fames
Manufactured by Merck Group
C8−C30 fatty acid methyl esters (FAMEs) are a series of saturated and unsaturated fatty acid methyl esters ranging from 8 to 30 carbon atoms in length. They are used as analytical standards and reference materials for the identification and quantification of fatty acids in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Hexane, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Methoxyamine hydrochloride, by Merck Group (2 mentions)
Pyridine, by Merck Group (2 mentions)
Advantage a10 system, by Merck Group (2 mentions)
N methyl n trimethylsilyltrifluoroacetamide mstfa, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Optima grade methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Absoluteidq p180 kit, by Biocrates (1 mentions)
Ethanol, by Merck Group (1 mentions)
2 protocols using c8 c30 fatty acid methyl esters fames
1
Metabolomic Analysis of Breast Cancer Cells
2
Comprehensive Metabolite Analysis Protocol
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