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Ag120

Manufactured by Selleck Chemicals

AG120 is a specialized laboratory instrument used for the analytical separation and purification of chemical substances. It employs a technique called affinity chromatography to selectively isolate target analytes from complex mixtures. The core function of AG120 is to facilitate the efficient and accurate separation and concentration of desired compounds for further analysis or purification.

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2 protocols using ag120

1

In Vivo Study Compound Formulation

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KPT-2974, AR-42, AG221, AG120, and PCI-34051 were purchased from Selleckchem. For in vivo study, AR-42 was formulated in 0.5% methylcellulose [w/v] and 0.1% Tween-80 [v/v] in sterile water.
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2

Kinase Activity Assay Protocol

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Myelin basic protein from bovine, DL-isocitric acid trisodium salt hydrate, β-nicotinamide adenine dinucleotide phosphate hydrate (NADP+), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), α-ketoglutaric acid sodium salt, diaphorase from Clostridium kluyveri, resazurin sodium salt, and ATP disodium salt hydrate were purchased from Sigma-Aldrich. Ketoglutaric acid sodium salt, α-[1-14C], 50μCi (1.85MBq) and glucose, D-[U-14C]- were from PerkinElmer. Glutamine L-[5-14C] and isocitric acid[3H(G)] were from ARC. Inhibitors including dovitinib (TKI-258, CHIR-258), imatinib, AG-490, quizartinib, PP2, erlotinib, ruxolitinib, dasatinib and AG-120 were purchased from Selleckchem. Recombinant proteins including FLT3, SRC, PDGFRα, PDGFRβ, EGFR, MET, KIT, JAK2, FGFR3 and ABL1 were purchased from Thermo Fisher.
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