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Anti β actin clone

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Anti-β-actin (clone) is a laboratory reagent used for the detection and quantification of the beta-actin protein, which is a fundamental component of the cytoskeleton in eukaryotic cells. This monoclonal antibody binds to the beta-actin protein and can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to analyze the expression and distribution of beta-actin in biological samples.

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Lab products found in correlation

Anti asc, by Santa Cruz Biotechnology (2 mentions) Anti human caspase 1, by Santa Cruz Biotechnology (2 mentions) Anti phospho s242 pyrin, by Abcam (2 mentions) Anti human gsgmd, by Merck Group (2 mentions) Mini protease inhibitor mixture, by Roche (2 mentions) Anti pyrin, by Adipogen (2 mentions) Transblot turbo midi size pvdf membranes, by Bio-Rad (2 mentions) Acrylamide gel, by Bio-Rad (2 mentions) Anti human il 1β, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (2 mentions) Anti flag m2 affinity gel, by Merck Group (2 mentions) Sodium fluoride, by Merck Group (2 mentions)

2 protocols using anti β actin clone

1

Pyrin-ASC Inflammasome Activation

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Cells were lysed in 25mM Tris HCl, 150mM NaCl, 1mM EDTA and 0.1% NP-40 buffer containing Mini Protease Inhibitor Mixture (Roche) and sodium fluoride (Sigma) by a quick freezing and thawing step. Flag-Pyrin was immuno-precipitated using anti Flag M2 affinity gel (Sigma). ASC was cross-linked in the insoluble pellet using DSS (Disuccinimidyl suberate, ThermoFisher #21655) 2 mM (1 h at 37°C). Proteins were separated by SDS/PAGE on precast 4–15% acrylamide gels (Bio-rad) and transferred to TransBlot® Turbo™ Midi-size PVDF membranes (Bio-rad). Antibodies used were mouse monoclonal anti-FLAG® (Sigma-Aldrich, clone M2; 1:1,000 dilution), anti-Pyrin (Adipogen, AL196, 1: 1,000 dilution), anti-phospho S242 Pyrin (Abcam, ab200420; 1:1,000 dilution) (Gao et al., 2016 (link)), anti-human Caspase-1 (Santa Cruz, sc515, 1: 1,000 dilution), anti-human GSGMD (sigma, HPA044487, 1: 1,000 dilution), anti-human IL-1β (Cell signaling, #12703, 1: 1,000 dilution), anti-ASC (Santa Cruz, sc22514R, 1:1,000 dilution). Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
Magnotti F., Chirita D., Dalmon S., Martin A., Bronnec P., Sousa J., Helynck O., Lee W., Kastner D.L., Chae J.J., McDermott M.F., Belot A., Popoff M., Sève P., Georgin-Lavialle S., Munier-Lehmann H., Tran T.A., De Langhe E., Wouters C., Jamilloux Y, & Henry T. (2022). Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell reports, 41(2), 111472.
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2

Pyrin-ASC Inflammasome Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 25mM Tris HCl, 150mM NaCl, 1mM EDTA and 0.1% NP-40 buffer containing Mini Protease Inhibitor Mixture (Roche) and sodium fluoride (Sigma) by a quick freezing and thawing step. Flag-Pyrin was immuno-precipitated using anti Flag M2 affinity gel (Sigma). ASC was cross-linked in the insoluble pellet using DSS (Disuccinimidyl suberate, ThermoFisher #21655) 2 mM (1 h at 37°C). Proteins were separated by SDS/PAGE on precast 4–15% acrylamide gels (Bio-rad) and transferred to TransBlot® Turbo™ Midi-size PVDF membranes (Bio-rad). Antibodies used were mouse monoclonal anti-FLAG® (Sigma-Aldrich, clone M2; 1:1,000 dilution), anti-Pyrin (Adipogen, AL196, 1: 1,000 dilution), anti-phospho S242 Pyrin (Abcam, ab200420; 1:1,000 dilution) (Gao et al., 2016 (link)), anti-human Caspase-1 (Santa Cruz, sc515, 1: 1,000 dilution), anti-human GSGMD (sigma, HPA044487, 1: 1,000 dilution), anti-human IL-1β (Cell signaling, #12703, 1: 1,000 dilution), anti-ASC (Santa Cruz, sc22514R, 1:1,000 dilution). Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
Magnotti F., Chirita D., Dalmon S., Martin A., Bronnec P., Sousa J., Helynck O., Lee W., Kastner D.L., Chae J.J., McDermott M.F., Belot A., Popoff M., Sève P., Georgin-Lavialle S., Munier-Lehmann H., Tran T.A., De Langhe E., Wouters C., Jamilloux Y, & Henry T. (2022). Steroid hormone catabolites activate the pyrin inflammasome through a non-canonical mechanism. Cell reports, 41(2), 111472.
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