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Inverted phase contrast microscopy

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The Inverted phase contrast microscopy is a type of optical microscope that uses phase contrast to produce high-contrast images of transparent specimens, such as living cells in culture. It allows for the observation of living cells and organisms without the need for staining or fixation, making it a valuable tool for biological research and cell culture applications.

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Phase-contrast microscopy (29 citations) Inverted microscopy (6 citations)

2 protocols using inverted phase contrast microscopy

1

Solamargine Cytotoxicity Assay

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The cells were treated with or without IC50 and IC70 concentrations of Solamargine or ≤0.1 % DMSO (as control) in medium without FBS. The morphological changes were observed after 2 and 24 h and photographed using inverted phase contrast microscopy (Zeiss, Germany).
Al Sinani S.S., Eltayeb E.A., Coomber B.L, & Adham S.A. (2016). Solamargine triggers cellular necrosis selectively in different types of human melanoma cancer cells through extrinsic lysosomal mitochondrial death pathway. Cancer Cell International, 16, 11.
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2

CuNPs and ACuNPs Cytotoxicity in MDA-MB 231 Cells

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MDA-MB 231 cells (5×105 cells/well) were seeded in 6-well plates and kept at 37°C, 5% CO2 for 24 h. Then, they were treated with IC50 of CuNPs and ACuNPs and again incubated for 24 h at 37 oC, 5% CO2. After the incubation of cells, the visible morphological changes in the cells were pictured under an inverted phase contrast microscopy (Zeiss, Germany).
Azizi M., Ghourchian H., Yazdian F., Dashtestani F, & AlizadehZeinabad H. (2017). Cytotoxic effect of albumin coated copper nanoparticle on human breast cancer cells of MDA-MB 231. PLoS ONE, 12(11), e0188639.
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