Poly l lysine coated culture vessels

Manufactured by ScienCell

Sourced in United States

Poly-L-lysine coated culture vessels are specialized laboratory equipment designed to facilitate cell culture and growth. The vessels are coated with the positively charged amino acid polymer, poly-L-lysine, which enhances cell adhesion and proliferation. This feature allows for improved cell attachment and growth within the culture environment.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions) Hek293a cells, by Thermo Fisher Scientific (1 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions) Poly l lysine coated culture vessels, by Corning (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Penicillin streptomycin, by ScienCell (1 mentions) Hbmec, by ScienCell (1 mentions) Endothelial cell medium (ecm), by ScienCell (1 mentions) Astrocyte medium, by ScienCell (1 mentions) Fibroblast medium, by ScienCell (1 mentions) Umuc3, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Nacalai Tesque (1 mentions)

2 protocols using poly l lysine coated culture vessels

Culturing Human Brain Cell Lines

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HEK293A cells (ThermoFisher Scientific) were maintained in DMEM (ThermoFisher Scientific), 10% fetal bovine serum (FBS) (ThermoFisher Scientific), 0.1 mM MEM non-essential amino acids (ThermoFisher Scientific) and subcultured every 3–5 days. Cells were used below 30 passages. Primary human brain microvascular endothelial cells (HBMEC) isolated from human brain tissue (ScienCell Research Laboratories, Carlsbad, CA) were cultured in endothelial cell growth medium (ECM, ScienCell) containing 5% FBS, 1% endothelial cell growth supplement and 100 units/ml penicillin/streptomycin in poly-L-lysine coated culture vessels (Corning Life Science, Tewksbury, MA). HBMEC cells were subcultured every 3 to 4 days and used between passages 5 and 10. Primary human cortical astrocytes isolated from fetal cerebral cortex (ScienCell) were cultured in astrocyte medium (ScienCell) containing 2% FBS, 1% astrocyte growth supplement and 100 units/ml penicillin/streptomycin in poly-L-lysine coated culture vessels and subcultured every 3 and 4 days and used between passages 5 and 10. All cells were cultured in a 37°C, 5% CO₂ incubator.

Zhang D., Kinloch A.J., Srinath A., Shenkar R., Girard R., Lightle R., Moore T., Koskimäki J., Mohsin A., Carrión-Penagos J., Romanos S., Shen L., Clark M.R., Shi C, & Awad I.A. (2020). Antibodies in Cerebral Cavernous Malformations React with Cytoskeleton Autoantigens in the Lesional Milieu. Journal of autoimmunity, 113, 102469.

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Urothelial Carcinoma Cell Lines: Cultivation and Characterization

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In this study, human urothelial carcinoma cell lines MGH-U-3, UM-UC-3, J82, and T24 were used. MGH-U-3 was received from Dr. H. LaRue (Laval University Cancer Research Centre, Quebec, Canada). UM-UC-3, J82, and T24 were bought from the American Type Culture Collection (ATCC, Manassas, VA, USA). MGH-U-3 was derived from bladder cancer with pathological Ta (pTa) grade1, UM-UC-3 was pT2-4, the grade was unknown, J82 was pT3 grade3, and T24 was pTa grade3. In a normal moisturized incubator at 37 °C with 5% CO₂, these cell lines were cultured in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) added with 10% fetal bovine serum (FBS; JRH, Tokyo, Japan) and 1% penicillin and streptomycin (Thermo Fisher Scientific, Yokohama, Japan). Human bladder stromal fibroblasts (HBdSF) and specific media were purchased from ScienCell Research Laboratories, Carlsbad, CA, USA. HBdSF were plated in poly-L-lysine-coated culture vessels to promote cell attachment (ScienCell Research Laboratories, Carlsbad, CA, USA) and cultured in fibroblast medium (ScienCell Research Laboratories) in a normal moisturized incubator at 37 °C with 5% CO₂.

Itami Y., Miyake M., Ohnishi S., Tatsumi Y., Gotoh D., Hori S., Morizawa Y., Iida K., Ohnishi K., Nakai Y., Inoue T., Anai S., Tanaka N., Fujii T., Shimada K., Furuya H., Khadka V.S., Deng Y, & Fujimoto K. (2020). Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder. Diagnostics, 10(1), 54.

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