Bovine milk casein

N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO), bovine serum albumin, N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), carboxypeptidase B, bovine milk casein, cysteine, EDTA, 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino) propane-1-sulfonic acid (MOPS), L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA), sinapic acid, N-Tris[hydroxymethyl]methyl-3aminopropanesulfonic acid (TAPS), Tris, and glycine were purchased from Sigma Chemical Company, St. Louis. Coomassie Brilliant Blue R-250, acrylamide, bisacrylamide and broad molecular weight markers were obtained from Bio-Rad, Hercules, CA. LMW-SDS markers kit, SP-Sepharose HR, Ampholytes Pharmalyte 8-10.5, and pI markers were purchased from GE Healthcare, Life Sciences, Uppsala, Sweden. All other chemicals were obtained from commercial sources and were of the highest purity available.

10 Bovine milk casein (C5890, Sigma-Aldrich, Saint Louis, MO, USA) was digested following the described procedure as a reference protein.
In vitro lipid digestibility was assessed in triplicate as reported by Capuano, Pellegrini, Ntone, and Nikiforidis (2018) , but using a pH of 7.7 and a reaction time of 20 min.
Lipid digestibility was calculated as the quantity of released fatty acids (expressed in oleic acid equivalents) over the total lipids (g/100 g lipids), taking into account the corrections calculated by analysing samples both without enzyme addition and defatted.
Extra virgin olive oil (Gaia, S. Rocco al Porto, LO, Italy) was digested following the described procedure as a reference fat.