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Pinpoint slide rna isolation system 2 kit

Manufactured by Zymo Research

The PinPoint Slide RNA Isolation System II kit is a tool designed for the extraction and purification of RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit utilizes a proprietary technology to capture and isolate RNA molecules from the tissue samples, allowing for downstream analysis and applications.

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2 protocols using pinpoint slide rna isolation system 2 kit

1

Nucleic Acid Isolation from Tissue Samples

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RNA was isolated using the Quick-RNA Miniprep Plus kit (Zymo Research) for fresh or frozen tissue specimens and the PinPoint Slide RNA Isolation System II kit (Zymo Research) for formalin-fixed, paraffin-embedded (FFPE)-preserved specimens. DNA was isolated from frozen tissue using the DNeasy Blood and Tissue Kit (Qiagen).
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2

FFPE RNA Extraction and qPCR Analysis

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FFPE PCa and normal tissue blocks were microdissected for RNA extraction using a Pinpointâ„¢ Slide RNA Isolation System II kit (Zymo Research, Orange, CA). RNA isolation from cells, cDNA synthesis and quantitative real-time polymerase chain reaction (qPCR) were performed as described [48 (link)]. Primers are listed in Supplementary Table 3.
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