Hygromycin

Manufactured by Gemini Bio

Sourced in Canada

Hygromycin is a laboratory reagent used as a selection marker in genetic engineering and cell culture experiments. It is an antibiotic that inhibits protein synthesis, allowing for the identification and isolation of successfully transfected or transformed cells.

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Lab products found in correlation

Recombinant human il1α protein, by R&D Systems (2 mentions) Ab16669, by BioLegend (2 mentions) Patm s1981, by Abcam (2 mentions) Af 400, by Active Motif (2 mentions) P p65 s536, by Cell Signaling Technology (2 mentions) P38 mapk inhibitor, by Selleck Chemicals (2 mentions) P p53 s15, by Cell Signaling Technology (2 mentions) γ h2ax, by Abcam (2 mentions) Sting, by Cell Signaling Technology (2 mentions) 4 hydroxytamoxifen, by Merck Group (2 mentions) Polybrene, by Merck Group (2 mentions) Puromycin, by Merck Group (2 mentions) Etoposide, by Merck Group (2 mentions) Luciferin, by PerkinElmer (2 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Mscv luciferase pgk hygro plasmid, by Addgene (1 mentions)

3 protocols using hygromycin

Innate Immunity and DNA Damage Response Pathway Analysis

Cited 1 time

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Etoposide, 4-hydroxytamoxifen, puromycin, and polybrene were purchased from Sigma. Hygromycin was from Gemini Bio Products, and luciferin was from PerkinElmer. Recombinant human IL1α protein (used at 20 ng/ml) was from R&D Systems, and p38 MAPK inhibitor from Selleck Chemicals.
The following antibodies were described previously: Flag, p16, Lamin B1, GAPDH, and GFP^{5 (link)}. Other antibodies used include: γH2AX (Abcam #ab2893 and Cell Signaling Technology #9718), STING (Cell Signaling Technology #13647 and LSBio #LS-B7237), cGAS (Cell Signaling Technology #15102 and Santa Cruz Biotechnology #sc-245858), human IL1α (Abcam #ab9614), IL8 (Abcam #ab18672), p-ATM S1981 (Abcam #ab81292), p-p53 S15 (Cell Signaling Technology #9284), p-p65 S536 (Cell Signaling Technology #3033), p65 (Cell Signaling Technology 8242), NRas (Santa Cruz Biotechnology #sc-31), CD3 (Abcam #ab16669), Mac2 (BioLegend #125401), mouse IL1α (R&D Systems #AF-400), p21 (#sc-471), and H3 (Active Motif #39763).

Dou Z., Ghosh K., Vizioli M.G., Zhu J., Sen P., Wangensteen K.J., Simithy J., Lan Y., Lin Y., Zhou Z., Capell B.C., Xu C., Xu M., Kieckhaefer J.E., Jiang T., Shoshkes-Carmel M., Ahasan Al Tanim K.M., Barber G.N., Seykora J.T., Millar S.E., Kaestner K.H., Garcia B.A., Adams P.D, & Berger S.L. (2017). Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature, 550(7676), 402-406.

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Innate Immunity and DNA Damage Response Pathway Analysis

Cited 1 time

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RFP and Luciferase Tagging of HPE-15 Cells

Cited 3 times

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Red fluorescence protein (RFP) tagging was performed by transfecting plasmid DNA (1 μg) of the expression vector pCDH-CMV-MSC-EF1-RFP-puro (CD516B-2, System Biosciences) following our reported protocol ^{6 (link), 7 (link), 14 (link)}. HPE-15^RFP, a representative clone of RFP-tagged HPE-15 cells after 2 weeks of puromycin (2 μg/ml, Life Technologies) selection and limiting dilution, was used in the study. To track xenograft tumor formation in NSG mice, HPE-15^RFP cells were further tagged with luciferase protein (luc) by transfection with the MSCV Luciferase PGK-hygro plasmid (Addgene, Cambridge, MA). HPE-15^RFP cells expressing luciferase, HPE-15^RFP/luc were selected with 200 μg/ml hygromycin (Gemini Bio-products, West Sacramento, CA).

Wang R., Chia-Yi Chu G., Wang X., Wu J.B., Hu P., Multani A.S., Pathak S., Zhau H.E, & Chung L.W. (2019). Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction. International journal of cancer, 145(8), 2249-2259.

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