The rates of apoptosis and survival were determined with FCM analysis by making use of an Annexin V‐APC/PI Apoptosis Assay kit (KeyGen Biotech) according to the instructions provided by the manufacturer. The reading of results was carried out on a FACS Canto II flow cytometer (BD Biosciences) utilizing the FlowJo X.10.0.7‐1 software (Bd Biosciences).
Annexin 5 apc pi apoptosis assay kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V-APC/PI apoptosis assay kit is a laboratory tool designed to detect and quantify apoptosis, a programmed cell death process. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a nucleic acid stain. This combination allows for the identification of cells undergoing early and late stages of apoptosis.
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4 protocols using annexin 5 apc pi apoptosis assay kit
1
Apoptosis Quantification by Flow Cytometry
2
Quantifying TE-1 Cell Apoptosis
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TE-1 cell apoptosis was determined using an Annexin V-APC/PI apoptosis assay kit (KeyGEN BioTECH, China) after transfection with SAA1 siRNA. Cells were digested with EDTA-free trypsin, subsequently washed with PBS, collected, and finally stained with Annexin V-APC and PI for 10 min in the dark. Cell apoptosis was analyzed using a flow cytometer (3L13C, ACEA Biosciences, USA).
3
Annexin V Apoptosis Assay of Gemcitabine-Treated Cells
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Cell apoptosis was analyzed using an Annexin V-APC/PI apoptosis assay kit (KeyGEN BioTECH). After being treated with or without gemcitabine for 48 h, cells were digested, washed, resuspended, and stained with Annexin V-APC and PI according to the manufacture's protocol. The apoptotic cells were detected with a flow cytometer (BD Biosciences).
4
Annexin V-APC/PI Apoptosis Assay
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The rates of apoptosis and survival were determined with FCM analysis by making use of an Annexin V-APC/PI Apoptosis Assay kit (KeyGen Biotech, Nanjing, China) according to the instructions provided by the manufacturer. The reading of results was done on a FACS Canto II ow cytometer (BD Biosciences, San Jose, CA) utilizing the FlowJo X.10.0.7-1 software.
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