Sk lms 1 human leiomyosarcoma cells

HT1080 human fibrosarcoma cells and SK-LMS-1 human leiomyosarcoma cells were obtained from the America Type Culture Collection (ATCC, Manassa, VA, USA). MS4515 and MS5907 mouse undifferentiated pleomorphic sarcoma cell lines were derived from genetically engineered mouse models, which we have described previously (Kirsch et al, 2007 (link)). All sarcoma cell lines were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U ml⁻¹ penicillin, 100 μg ml⁻¹ streptomycin, and L-glutamine 2 mM. Human cancer cell lines were actively passaged for <6 months from the time that they were received from ATCC, and United Kingdom Co-ordinating Committee on Cancer Research (UKCCCR) guidelines were followed (UKCCCR, 2000 (link)). Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (Basel, Switzerland) and used within eight passages. All endothelial cells were grown in EGM-2-MV media (Lonza).
The anti-VEGFR2 antibody DC101 was purchased from Bio X Cell (West Lebanon, NH, USA). Immunoglobulin G antibody was purchased from Sigma-Aldrich (St Louis, MO, USA). Doxorubicin was purchased from Teva Pharmaceuticals (Tikva, Israel). TH-302 was obtained from Threshold Pharmaceuticals (South San Francisco, CA, USA).