Human mda mb 231 bc cell line

Mouse 4T1 and human UACC812 BC cell lines were purchased from the American Type Culture Collection (ATCC) and the Human MDA-MB-231 BC cell line was acquired from the Duke Comprehensive Cancer Center Cell Culture Facility. Human MDA-MB-231 and UACC812 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% heat-inactivated Gemini Foundation fetal bovine serum (FBS; Gemini Bioproducts, LLC, West Sacramento, CA, USA). The mouse HER2/neu + MCa-M3C cell line, developed at Massachusetts General Hospital, was cultured in DMEM supplemented with 15% heat-inactivated FBS[27 (link)]. Mouse triple-negative 4T1 cells[28 (link)] were cultured in RPMI 1640 medium (Corning) supplemented with 10% FBS. All the cells were cultured at 37°C in a 5% CO₂ atmosphere.

Mouse 4T1 and human UACC812 BC cell lines were purchased from the American Type Culture Collection (ATCC) and the Human MDA-MB-231 BC cell line was acquired from the Duke Comprehensive Cancer Center Cell Culture Facility. Human MDA-MB-231 and UACC812 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% heat-inactivated Gemini Foundation fetal bovine serum (FBS; Gemini Bioproducts, LLC, West Sacramento, CA, USA). The mouse HER2/neu⁺ MCa-M3C cell line, was developed at Massachusetts General Hospital, and cultured in DMEM supplemented with 15% heat-inactivated FBS [28 (link)]. The mouse triple-negative 4T1 cells [29 (link)] were cultured in RPMI 1640 medium (Corning) supplemented with 10% FBS. All the cells were cultured at 37 °C in a 5% CO₂ atmosphere.

The Hep3B human HCC cell line and MDA-MB-231 human BC cell line were purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA). MDA-MB-231 cells were grown in RPMI-1640 medium (WelGENE, Daegu, Korea) supplemented with 100 mL/L of fetal bovine serum (FBS; WelGENE), 100,000 U/L of penicillin (WelGENE), and 100 mg/L of streptomycin (WelGENE). Hep3B cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, WelGENE) supplemented as described above. The cells were cultured at 37 °C in a humidified atmosphere under 5% CO₂.