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Igrov

Manufactured by American Type Culture Collection
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The IGROV is a laboratory equipment designed for the storage and maintenance of cell cultures. It provides a controlled environment for the preservation and growth of various cell lines. The IGROV operates within a specified temperature range and incorporates features to ensure the viability and integrity of the stored cell cultures.

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Lab products found in correlation

Skov3, by American Type Culture Collection (2 mentions) Ovcar3, by American Type Culture Collection (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Rnaimax, by Thermo Fisher Scientific (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) A2780, by American Type Culture Collection (2 mentions) Ovca432, by American Type Culture Collection (2 mentions) Heya8, by American Type Culture Collection (2 mentions) Gentamicin sulfate, by Gemini Bio (2 mentions) Rpmi 1640, by Gemini Bio (2 mentions) Gilm2, by American Type Culture Collection (2 mentions) Acetonitrile, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by American Type Culture Collection (1 mentions) Methyl thiazolyl tetrazolium (mtt), by American Type Culture Collection (1 mentions) Dulbecco s modified eagle s medium, by American Type Culture Collection (1 mentions) Acetic acid, by Merck Group (1 mentions) Gallic acid, by American Type Culture Collection (1 mentions) Ovcar, by American Type Culture Collection (1 mentions)

4 protocols using igrov

1

Protocols for Culturing and Transfecting Ovarian and Breast Cancer Cell Lines

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All cell lines were maintained in 5% CO2 at 37°C. Ovarian cancer (A2780, OVCAR3, SKOV3, OVCA432, HeyA8, IGROV, EG) and breast cancer (MDA-MB-231, MCF7, GILM2) cells were obtained from the American Type Culture Collection and were maintained in RPMI 1640 supplemented with 10–15% fetal bovine serum (FBS) and 0.1% gentamicin sulfate (GeminiBioproducts, Calabasas, CA). All cell lines were routinely tested to confirm the absence of Mycoplasma, and all in vitro experiments were conducted with 60–80% confluent cultures.
All siRNA transfections (Supplementary Table 1) were performed using RNAi MAX (Invitrogen Carlsbad, CA) reagent using forward transfection protocol from the manufacturer. Media was changed 5 hours after transfections to minimize toxicity. For all hypoxia treatments, cells were incubated in an oxygen-controlled hypoxia chamber at 1% O2. For ectopic expression of Drosha and Dicer, we obtained plasmids from Addgene (IDs 10828, 25851 respectively). Next, we cloned open reading frames into pLKO.1-GFP or Puromycin lentiviral plasmids. We transduced HeyA8 cells with virus particles and then selection using GFP (Drosha) or puromycin (Dicer) was carried out to establish stable cell variants.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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2

Protocols for Culturing and Transfecting Ovarian and Breast Cancer Cell Lines

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All cell lines were maintained in 5% CO2 at 37°C. Ovarian cancer (A2780, OVCAR3, SKOV3, OVCA432, HeyA8, IGROV, EG) and breast cancer (MDA-MB-231, MCF7, GILM2) cells were obtained from the American Type Culture Collection and were maintained in RPMI 1640 supplemented with 10–15% fetal bovine serum (FBS) and 0.1% gentamicin sulfate (GeminiBioproducts, Calabasas, CA). All cell lines were routinely tested to confirm the absence of Mycoplasma, and all in vitro experiments were conducted with 60–80% confluent cultures.
All siRNA transfections (Supplementary Table 1) were performed using RNAi MAX (Invitrogen Carlsbad, CA) reagent using forward transfection protocol from the manufacturer. Media was changed 5 hours after transfections to minimize toxicity. For all hypoxia treatments, cells were incubated in an oxygen-controlled hypoxia chamber at 1% O2. For ectopic expression of Drosha and Dicer, we obtained plasmids from Addgene (IDs 10828, 25851 respectively). Next, we cloned open reading frames into pLKO.1-GFP or Puromycin lentiviral plasmids. We transduced HeyA8 cells with virus particles and then selection using GFP (Drosha) or puromycin (Dicer) was carried out to establish stable cell variants.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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3

Evaluation of Bioactive Compounds

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All chemicals used were of analytical reagent grade. All reagents were purchased from Sigma Aldrich (France): acetic acid, acetonitrile (ACN), cyclohexane (CYHA), dichloromethane (DCM), methanol (MeOH), Dulbecco's modified Eagle's medium (DMEM), dimethyl sulfoxide (DMSO), tamoxifen, Folin-Ciocalteu reagent (2 N), gallic acid, quercetin, catechin, HCl, KH2PO4, MTT, NaOH, sodium carbonate, 15-LOX, and AChE. Cell lines (OVCAR and IGROV) were purchased from American Type Culture Collection (USA).
Worowounga X., Rahmani R., Namkona A.F., Cazaux S., Syssa-Magalé J.L., Matondo H, & Bouajila J. (2022). Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities. International Journal of Analytical Chemistry, 2022, 4066783.
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Cytotoxicity of M. mabokeensis Bark Extracts

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The cytotoxicity of the different M. mabokeensis Aubrév bark extracts was estimated on IGROV and OVCAR cells lines (American Type Culture Collection) as described by Kohoude et al. [14 (link)], with minor modifications. Cells were distributed in 96-well plates at 3 × 104 cells/well in 100 μL. After that, 100 μL of the corresponding culture medium (DMEM) containing sample at various concentrations was added. Cell growth was estimated by the MTT assay. MTT is a water-soluble tetrazolium salt with a yellow coloration. Metabolically active cells are able to convert the dye to water-insoluble dark blue formazan by reductive cleavage of the tetrazolium ring. The extracts were resolubilized in the DMSO followed by dilution in the buffer, whereby the DMSO does not exceed 1%. Doxorubicin was used as a positive control. The cells activity inhibition percentage was calculated as follows: % inhibition=100×Ablank − AsampleAblank.
Worowounga X., Rahmani R., Namkona A.F., Cazaux S., Syssa-Magalé J.L., Matondo H, & Bouajila J. (2022). Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities. International Journal of Analytical Chemistry, 2022, 4066783.
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