MDA-MB-231 human breast adenocarcinoma (American Type Culture Collection) were maintained at sub-confluent densities with 5% CO2 at 37°C in DMEM supplemented with 10% fetal bovine serum and 1X antibiotic/antimycotic (Life Technologies).
Mda mb 231 human breast adenocarcinoma
Manufactured by American Type Culture Collection
MDA-MB-231 is a human breast adenocarcinoma cell line derived from a pleural effusion. It is a widely used model for studying breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Penicillin strep, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
A375 human melanoma, by American Type Culture Collection (1 mentions)
A549 human lung adenocarcinoma, by American Type Culture Collection (1 mentions)
2 protocols using mda mb 231 human breast adenocarcinoma
1
Culturing MDA-MB-231 Breast Cancer Cells
2
Cell Culture Conditions for Cancer Cell Lines
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A375 human melanoma, A549 human lung adenocarcinoma, and MDA-MB-231 human breast adenocarcinoma cell lines were purchased from the American Type Culture Collection (ATCC); HaCaT-human immortalized keratinocyte cell line was provided by the University of Debrecen, Hungary as a kind gift.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
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