Primary antibodies detecting anti-human cleaved caspase-8 (Asp391) (18C8; #9496), anti-mouse cleaved caspase-8 (Asp387) (D5B2; #8592), anti-human and -mouse cleaved caspase-3 (Asp175) (5A1E; #9664), anti-human cleaved PARP (D64E10; #5625) were purchased from Cell Signaling Technology®, anti-human CD68 (PG-M1; #M0876) from Dako and anti-mouse Iba-1 (#NB100-1028) from Novus Biologicals and (#019-19741) from Wako. For immunofluorescence detection, fluorophore conjugated secondary antibodies were purchased from Invitrogen, ThermoFisher Scientific. Goat anti-rabbit Alexa Fluor®488 and goat anti-mouse Alexa Fluor®594 were used for the human tissue analysis, whereas donkey anti-rabbit Alexa Fluor®488 and donkey anti-goat Alexa Fluor®594 were used for mouse tissue analysis. Biotinylated goat anti-rabbit IgG were obtained from Vector Laboratories.
Anti human cd68 pg m1
Manufactured by Agilent Technologies
Sourced in Switzerland
The Anti-human CD68 (PG-M1) is a laboratory equipment product from Agilent Technologies. It is a monoclonal antibody that specifically binds to the CD68 antigen expressed on human macrophages and monocytes. The core function of this product is to enable the detection and identification of cells expressing the CD68 antigen through immunohistochemical or flow cytometric analysis.
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2 protocols using anti human cd68 pg m1
1
Immunodetection of Apoptosis Markers
2
Multicolor Flow Cytometry and IHC Analysis
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Antibodies used for flow cytometric analysis were PEconjugated anti-CD115 (9-4D2-1E4), PerCP/Cy5.5-conjugated anti-HLA-DR (L243), APC-conjugated anti-CD68 (Y1/82A), PE/Cy7conjugated anti-CD14 (HCD14) and Brilliant Violet 421conjugated anti-CD45 from Biolegend (UK). PerCP/Cy5.5 antialkaline phosphatase (B4-78) was from BD Pharmingen (USA). FITC-conjugated anti-CD45 (HI30) was from Tonbo Biosciences (USA). The mouse monoclonal antibodies used for immunohistochemical analysis were anti-human CD68 (PG-M1, Dako, Switzerland) and anti-human osteocalcin (OCG3, Abcam, UK).
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