RNA was isolated from sorted cells using the Promega RNA Reliprep kit. cDNA was synthesized and amplified using the Ovation PicoSL WTA V2 kit (NuGen). RT-PCR reactions were performed using Faststart Universal SYBR green Master Mix (Roche) and run on an Eppendorf Realplex2 (link) thermocycler. Primer sequences are as listed:
Rna reliprep kit
Manufactured by Promega
The RNA Reliprep kit is a product designed for the rapid and efficient extraction of high-quality RNA from a variety of sample types. The kit utilizes a specialized resin-based technology to capture and purify RNA molecules, enabling researchers to obtain reliable and reproducible results for downstream applications.
Automatically generated - may contain errors
2 protocols using rna reliprep kit
1
RNA Isolation and RT-PCR Analysis
2
RNA Isolation and RT-PCR Analysis
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